Autofluorescence Imaging to Evaluate Cellular MetabolismAnna Theodossiou 1, Linghao Hu 1, Nianchao Wang 1, Uyen Nguyen 1, Alex J. Walsh 1
1Department of Biomedical Engineering, Texas A&M University-College Station
This protocol describes fluorescence imaging and analysis of the endogenous metabolic coenzymes, reduced nicotinamide adenine (phosphate) dinucleotide (NAD(P)H), and oxidized flavin adenine dinucleotide (FAD). Autofluorescence imaging of NAD(P)H and FAD provides a label-free, nondestructive method to assess cellular metabolism.
