Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson's Disease ModelNaemah Md Hamzah 1, Siong Meng Lim 1, Yuganthini Vijayanathan 1,2, Fei Tieng Lim 1, Abu Bakar Abdul Majeed 1, Maw Pin Tan 2, Kalavathy Ramasamy 1
1Collaborative Drug Discovery Research (CDDR) Group and Brain Degeneration and Therapeutics Group, Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Cawangan Selangor, Kampus Puncak Alam, 2Department of Medicine, Faculty of Medicine, Universiti Malaya
The present protocol describes the intracerebroventricular (ICV) injection of adult zebrafish with neurotoxic 6-hydroxydopamine (6-OHDA) at the ventral diencephalon (Dn) and the assessment of the impairment and subsequent recovery of swimming behavior postlesion by using the open tank test, which is accompanied by analysis using a video tracking software.
