Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae CellsHimanshu Pandey 1, Tatiana Zvagelsky 1, Mary Popov 1, Mayan Sadan 1, Neta Yanir 1, Alina Goldstein-Levitin 1, Nurit Siegler 1, Shira Hershfinkel 1, Yahel Abraham 1, Roy Avraham 1, Levi A. Gheber 2, Larisa Gheber 1
1Department of Chemistry, Ben-Gurion University of the Negev, Israel, 2Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Israel
The bi-directional mitotic kinesin-5 Cin8 accumulates in clusters that split and merge during their motility. Accumulation in clusters also changes the velocity and directionality of Cin8. Here, a protocol for motility assays with purified Cin8-GFP and analysis of motile properties of single molecules and clusters of Cin8 is described.
