Chengdu Women’s and Children's Central Hospital

2 ARTICLES PUBLISHED IN JoVE

Medicine

Blood Circuit Reconstruction in an Abdominal Mouse Heart Transplantation Model

Decheng Yin^*1,2, Jian Fu^*1,3, Ida Allabauer¹, Oliver Witzke⁴, Song Rong⁵, André Hoerning¹

¹Department of Pediatric and Adolescent Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, ²Department of Pediatric Surgery, Chengdu Women's and Children's Central Hospital, ³The Key Laboratory of Hainan Trauma and Disaster Rescue, The first affiliated Hospital of Hainan Medical University, ⁴Department of Infectious Diseases, West German Centre of Infectious Diseases, Universitätsmedizin Essen, University Duisburg-Essen, ⁵Department of Nephrology, Hannover Medical School

A novel technique for blood circuit reconstruction in a heterotopic abdominal mouse heart transplantation model is demonstrated.

Immunology and Infection

A Modified Surgical Technique for Kidney Transplantation in Mice

Decheng Yin^*1,2, Jian Fu^*1,3, Rongjun Chen⁴, Nelli Shushakova⁵, Ida Allabauer¹, Xin-Yi Wei¹, Mario Schiffer⁶, Diana Dudziak⁷, Song Rong^*5, André Hoerning^*1

¹Department of Pediatric and Adolescent Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, ²Department of Pediatric Surgery, Chengdu Women’s and Children's Central Hospital, ³The Key Laboratory of Hainan Trauma and Disaster Rescue, The first affiliated Hospital of Hainan Medical University, ⁴Department of Department of Pediatric Kidney, Liver, and Metabolic Diseases, Hannover Medical School, ⁵Department of Nephrology, Hannover Medical School, ⁶Department of Nephrology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, ⁷Department of Dermatology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg

This protocol presents a new surgical technique of mouse kidney transplantation focusing on a modified arterial anastomosis strategy. A vascular suture technique including a simple and safer ureter-bladder anastomosis method is also presented. These modifications shorten the operation time and improve the success rate of the mouse kidney transplantation procedure.