Natural Transformation, Protein Expression, and Cryoconservation of the Filamentous Cyanobacterium Phormidium lacunaNora Weber 1, Michael Hofmeister 1, Nadja Wunsch 1, Anja Kohler 1, Anne-Kristin Kaster 2, Jon Vollmers 2, Ben Kachel 3, Matthias Mack 3, Tilman Lamparter 1
1Botanical Institute, Karlsruhe Institute of Technology KIT, 2Institute for Biological Interfaces 5 (IBG 5), Karlsruhe Institute of Technology KIT, 3Institut für Technische Mikrobiologie, Hochschule Mannheim
Phormidium lacuna is a filamentous cyanobacterium that was isolated from marine rockpools. This article describes the isolation of filaments from natural sources, DNA extraction, genome sequencing, natural transformation, expression of sfGFP, cryoconservation, and motility methods.
