Institute of Pathology Laenggasse

2 ARTICLES PUBLISHED IN JoVE

Medicine

The Rabbit Blood-shunt Model for the Study of Acute and Late Sequelae of Subarachnoid Hemorrhage: Technical Aspects

Lukas Andereggen^3,4,5, Volker Neuschmelting^1,6, Michael von Gunten⁷, Hans Rudolf Widmer⁵, Jukka Takala¹, Stephan M. Jakob¹, Javier Fandino^1,2, Serge Marbacher^1,2

¹Department of Intensive Care Medicine, University and Bern University Hospital (Inselspital), ²Department of Neurosurgery, Kantonsspital Aarau, ³Laboratories for Neuroscience Research in Neurosurgery, Boston Children's Hospital, ⁴Harvard Medical School, Boston Children's Hospital, ⁵Department of Neurosurgery, University and Bern University Hospital (Inselspital), ⁶Department of Neurosurgery, University Hospital Cologne, ⁷Institute of Pathology, Länggasse Bern

The experimental intracranial pressure-controlled blood shunt subarachnoid hemorrhage (SAH) model in the rabbit combines the standard procedures — subclavian artery cannulation and transcutaneous cisterna magna puncture, which enables close mimicking of human pathophysiological conditions after SAH. We present step-by-step instructions and discuss key surgical points for successful experimental SAH creation.

Neuroscience

Using a Cell-Tracer Injection to Investigate the Origin of Neointima-Forming Cells in a Rat Saccular Side Wall Model

Stefan Wanderer^*1,2, Basil E. Grüter^*1,2, Jeannine Kümin^1,2, Gwendoline Boillat^1,2, Sivani Sivanrupan², Kristina Catalano^1,2, Michael von Gunten³, Hans Rudolf Widmer⁴, Serge Marbacher^1,2,5, Lukas Andereggen^1,2,5

¹Department of Neurosurgery, Kantonsspital Aarau, ²Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, ³Institute of Pathology Laenggasse, ⁴Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Inselspital, Bern University Hospital, University of Bern, ⁵Faculty of Medicine, University of Bern

We performed a one-point, lipophilic cell-tracer injection to track endothelial cells, followed by an arteriotomy and suturing of sidewall aneurysms on the abdominal rat aorta. Neointima formation seemed dependent on the parent artery in decellularized aneurysms and was promoted by the recruitment from aneurysm wall cells in vital cell-rich walls.