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USDA-ARS Cattle Fever Tick Research Laboratory

1 ARTICLES PUBLISHED IN JoVE

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Biology

Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles
Brenda Leal-Galvan 1, Cristina Harvey 1, Donald Thomas 2, Perot Saelao 3, Adela S. Oliva Chavez 1
1Department of Entomology, Texas A&M University, 2USDA-ARS Cattle Fever Tick Research Laboratory, 3USDA-ARS Veterinary Pest Research Unit

The present protocol describes the isolation of microRNAs from tick salivary glands and purified extracellular vesicles. This is a universal procedure that combines commonly used reagents and supplies. The method also allows the use of a small number of ticks, resulting in quality microRNAs that can be readily sequenced.

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