Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III CNIC

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Inner Mitochondrial Membrane Sensitivity to Na⁺ Reveals Partially Segmented Functional CoQ Pools

Pablo Hernansanz-Agustín¹, José Antonio Enríquez^1,2

¹Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III CNIC, ²Centro de Investigaciones Biomédica en Red de Fragilidad y Envejecimiento, Saludable-CIBERFES

This protocol describes a comparative assay, using mitochondrial complex activities CI+CIII and CII+CIII in the presence or absence of Na⁺, to study the existence of partially segmented functional CoQ pools.