Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family MotorsPushpanjali Soppina 1,2, Dipeshwari J. Shewale 1, Pradeep K. Naik 2, Virupakshi Soppina 1
1Discipline of Biological Engineering, Indian Institute of Technology Gandhinagar, 2Department of Biotechnology and Bioinformatics, Sambalpur University
This study details purification of KIF1A(1-393LZ), a member of kinesin-3 family, using Sf9-baculovirus expression system. In vitro single-molecule and multi-motor gliding analysis of these purified motors exhibited robust motility properties comparable to motors from mammalian cell lysate. Thus, Sf9-baculovirus system is amenable to express and purify motor protein of interest.
