Sign In

University of Cambridge, Cambridge Biomedical Campus

2 ARTICLES PUBLISHED IN JoVE

image

Medicine

Measuring Single-Cell Mitochondrial DNA Copy Number and Heteroplasmy Using Digital Droplet Polymerase Chain Reaction
Stephen P. Burr 1,2, Patrick F. Chinnery 1,2
1Department of Clinical Neurosciences, School of Clinical Medicine, University of Cambridge, Cambridge Biomedical Campus, 2Medical Research Council Mitochondrial Biology Unit, University of Cambridge, Cambridge Biomedical Campus

Here we present a protocol for measuring absolute mitochondrial (mt)DNA copy number and mtDNA deletion heteroplasmy levels in single cells.

image

Medicine

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Pavel A. Nash 1, Pedro Silva-Pinheiro 1, Michal A. Minczuk 1
1Medical Research Council Mitochondrial Biology Unit, University of Cambridge, Cambridge Biomedical Campus

Pyrosequencing assays enable the robust and rapid genotyping of mitochondrial DNA single nucleotide polymorphisms in heteroplasmic cells or tissues.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved