Genetic Screen for Identification of Multicopy Suppressors in Schizosaccharomyces pombeVaibhav Bhardwaj *1, Kumari Sweta *1, Deepak Gyala 1, Nimisha Sharma 1
1University School of Biotechnology, G.G.S. Indraprastha University
This work describes a protocol for a multicopy suppressor genetic screen in Schizosaccharomyces pombe. This screen uses a genome-wide plasmid library to identify suppressor clone(s) of a loss-of-function phenotype associated with a query mutant strain. Novel genetic suppressors of the ell1 null mutant were identified using this screen.
