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San Diego Zoo Wildlife Alliance

1 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
Laura Adams *1, Ying Liu *1, Barbara Durrant 2, Elena Ruggeri 2, Carly Young 2, Rebecca Krisher 3, Irina Polejaeva 1
1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 2Reproductive Sciences, Beckman Center for Conservation Research, San Diego Zoo Wildlife Alliance, 3Genus PLC

Here, we present a protocol to significantly reduce the mitochondrial DNA copy numbers in a bovine oocyte (P < 0.0001). This method utilizes centrifugation and bisection to substantially reduce oocyte mitochondria and may allow for an increased chance of development in the reconstructed interspecies somatic cell nuclear transfer embryos.

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