Spanish Biomedical Research Network in Physiopathology of Obesity and Nutrition (CIBEROBN)

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Enrichment of Astrocyte-Derived Extracellular Vesicles from Human Plasma

Natalia Valle-Tamayo^1,2, Rocío Pérez-González^2,3, Gemma Chiva-Blanch^4,5, Olivia Belbin^1,2, Sara Serrano-Requena^1,2, Sònia Sirisi^1,2, Alba Cervantes González^1,2, Oriol Giró^4,5, Érika Sánchez-Aced^1,2, Oriol Dols-Icardo^1,2, Daniel Alcolea^1,2, María Carmona-Iragui^1,2, Amanda Jimenez^4,5, Alberto Lleó^1,2, Juan Fortea^1,2, M. Florencia Iulita^1,2

¹Sant Pau Memory Unit, Department of Neurology, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau, Universitat Autònoma de Barcelona, ²Center of Biomedical Investigation Network for Neurodegenerative Diseases (CIBERNED), ³Movement Disorders Unit, Department of Neurology, Hospital de la Santa Creu i Sant Pau, ⁴Department of Endocrinology and Nutrition, Hospital Clínic de Barcelona, August Pi i Sunyer Biomedical Research Institute - IDIBAPS, ⁵Spanish Biomedical Research Network in Physiopathology of Obesity and Nutrition (CIBEROBN)

This protocol describes the enrichment of astrocyte-derived extracellular vesicles (ADEVs) from human plasma. It is based on the separation of EVs by polymer precipitation, followed by ACSA-1 based immunocapture of ADEVs. Analysis of ADEVs may offer clues to study changes in inflammatory pathways of living patients, non-invasively by liquid biopsy.