Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene EditingMathilde Beaufils 1, Amandine Tourel 1, Anne Petiot 1, Nicole B. Halmai 2, Dave J. Segal 2, John Rendu 1, Isabelle Marty 1
1University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, 2Genome Center, University of California, Davis
The protocol describes how to generate knock-out myoblasts using CRISPR/Cas9, starting from the design of guide-RNAs to the cellular cloning and characterization of the knock-out clones.