Indian Institute of Science Education and Research

1 ARTICLES PUBLISHED IN JoVE

Cancer Research

Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures

Abhijith Kuttanamkuzhi¹, Libi Anandi^1,2, Vaishali Chakravarty^1,3, Mayurika Lahiri¹

¹Biology Department, Indian Institute of Science Education and Research, ²Department of Biology, Center for Genomics and Systems Biology, New York University, ³Enveda Therapeutics India Pvt Ltd.

The present protocol describes the setting up of 3D 'on top' cultures of a non-transformed breast epithelial cell line, MCF10A, that has been modified to study Platelet Activating Factor (PAF) induced transformation. Immuno-fluorescence has been used to assess the transformation and is discussed in detail.