Live Imaging of Early Cardiac Progenitors in the Mouse EmbryoMiquel Sendra 1, Jorge Mañes 1, Jorge N. Domínguez 2,3, Miguel Torres 1
1Cardiovascular Regeneration Program, Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Cardiovascular Development Group, Department of Experimental Biology, University of Jaén, Jaén, 3Fundación Medina, Granada
We present a detailed protocol for mouse embryo culture and imaging that enables 3D + time imaging of cardiac progenitor cells. This video-toolkit addresses the key skills required for successful live imaging otherwise hard to acquire from text-only publications.