Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome SequencingJosé Antonio Magaña-Lizárraga 1, Bruno Gómez-Gil 2, Julissa Enciso-Ibarra 2, María Elena Báez-Flores 1
1Unidad de Investigaciones en Salud Pública “Dra. Kaethe Willms”, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, 2Centro de Investigación en Alimentación y Desarrollo, A. C. (CIAD), Unidad Mazatlán en Acuicultura y Manejo Ambiental
The feasibility of whole-genome sequencing (WGS) strategies using benchtop instruments has simplified the genome interrogation of every microbe of public health relevance in a lab setting. A methodological adaptation of the workflow for bacterial WGS is described and a bioinformatics pipeline for analysis is also presented.