Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiaeJuan de Dios Barba Tena 1, Etienne Schwob 1, Nicolas Talarek 1
1Institut de Génétique Moléculaire de Montpellier, Université de Montpellier, CNRS
We describe two complementary protocols to accurately determine S-phase duration in S. cerevisiae using EdU, a thymidine analog, which is incorporated in vivo and detected using Click chemistry by microscopy and flow cytometry. It allows for the easy characterization of the duration of DNA replication and overlooked replication defects in mutants.
