Bioengineering College of Chongqing University

7 ARTICLES PUBLISHED IN JoVE

Medicine

Programmed Electrical Stimulation in Mice

¹Department of Molecular Physiology and Biophysics, Baylor College of Medicine (BCM), ²The Margaret M. and Albert B. Alkek Department of Medicine, Baylor College of Medicine (BCM)

Programmed electrical stimulation provides the ability to determine conduction properties of the heart, and the possibility to induce and terminate cardiac arrhythmias using various pacing protocols. Using a transvenous catheter, intracardiac electrogram recordings can be obtained in mice following programmed electrical stimulation protocols to identify arrhythmogenic substrates.

Biology

Live Cell Imaging of Primary Rat Neonatal Cardiomyocytes Following Adenoviral and Lentiviral Transduction Using Confocal Spinning Disk Microscopy

Takashi Sakurai^1,2, Anthony Lanahan², Melissa J. Woolls², Na Li², Daniela Tirziu², Masahiro Murakami²

¹Max-Planck-Institute for Molecular Biomedicine and Institute of Cell Biology, ²Department of Internal Medicine, Yale Cardiovascular Research Center and Section of Cardiovascular Medicine

This protocol describes a method of live cell imaging using primary rat neonatal cardiomyocytes following lentiviral and adenoviral transduction using confocal spinning disk microscopy. This enables detailed observations of cellular processes in living cardiomyocytes.

Biology

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay

Courtney M. Anderson¹, Annelies Laeremans¹, Xiao-Ming Mindy Wang¹, Xingyong Wu¹, Bingqing Zhang¹, Emerald Doolittle¹, Jeffrey Kim¹, Na Li¹, Helly Xiao Yan Pimentel¹, Emily Park¹, Xiao-Jun Ma¹

¹Advanced Cell Diagnostics, Inc

Here, we describe an in situ hybridization assay which enables sensitive and specific detection of sequences as short as 50 nucleotides with single-nucleotide resolution at the single-cell level. The assay, which can be performed manually or automatically, can enable visualization of splice variants, short sequences, and mutations within the tissue context.

Cancer Research

Preparation of Mitochondria from Ovarian Cancer Tissues and Control Ovarian Tissues for Quantitative Proteomics Analysis

Xianquan Zhan^1,2,3,4, Huanni Li⁵, Shehua Qian^1,2,3, Xiaohan Zhan^1,2,3, Na Li^1,2,3

¹Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, ²Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital, Central South University, ³State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital, Central South University, ⁴National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, ⁵Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University

This article presents a protocol of differential-speed centrifugation in combination with density gradient centrifugation to separate mitochondria from human ovarian cancer tissues and control ovarian tissues for quantitative proteomics analysis, resulting in a high-quality mitochondrial sample and high-throughput and high-reproducibility quantitative proteomics analysis of a human ovarian cancer mitochondrial proteome.

Cancer Research

Analysis of Side Population in Solid Tumor Cell Lines

Xiaoli Dong¹, Yingying Wei¹, Tao Xu¹, Xiaoyue Tan^1,2,3, Na Li^1,2,3

¹School of Medicine, Nankai University, ²Tianjin Key Laboratory of Tumour Microenvironment and Neurovascular Regulation, ³Collaborative Innovation Center for Biotherapy, Nankai University

A convenient, fast, and cost-effective method to measure the proportion of side population cells in solid tumor cell lines is presented.

Medicine

Investigating the Protective Effects of Platycodin D on Non-Alcoholic Fatty Liver Disease in a Palmitic Acid-Induced In Vitro Model

Xingjian Wen^*1,2, Jia Wang^*1, Jiuyu Fan¹, Rui Chu¹, Yilong Chen¹, Yajing Xing¹, Na Li^1,2, Guixue Wang²

¹Chongqing Academy of Chinese Materia Medica, ²Bioengineering College of Chongqing University

This protocol investigates the protective effects of platycodin D on non-alcoholic fatty liver disease in a palmitic acid-induced in vitro model.

Medicine

Optimization of the Epimedii Folium Mutton-Oil Processing Technology and Testing Its Effect on Zebrafish Embryonic Development

Jiuyu Fan¹, Xingjian Wen^1,2, Shengrong Li¹, Rui Chu¹, Yilong Chen¹, Zhimin Su¹, Na Li^1,2

¹Chongqing Academy of Chinese Materia Medica, ²Bioengineering College of Chongqing University

In this protocol, the mutton-oil processing technology of Epimedii folium (EF) was optimized by applying a Box-Behnken experimental design-response surface methodology, and the effect of crude and optimized water-extracted EF on zebrafish embryonic development was preliminarily investigated.