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Peak Proteins Ltd.

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography
Ewa Rejnowicz 1, Jack Wright 1, Margaret Veldman-Jones 1, Steven Harborne 1
1Peak Proteins Ltd.

The present protocol describes a procedure to perform fluorescent size exclusion chromatography (FSEC) on membrane proteins to assess their quality for downstream functional and structural analysis. Representative FSEC results collected for several G-protein coupled receptors (GPCRs) under detergent-solubilized and detergent-free conditions are presented.

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