Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution RespirometryTessa C. Fitch *1,2, Scott I. Frank *1, Yutong Kelly Li 1, Magali Saint-Geniez 1,2, Leo A. Kim 1,2, Daisy Y. Shu 1,2
1Schepens Eye Research Institute of Mass. Eye and Ear, 2Department of Ophthalmology, Harvard Medical School
The metabolic status of human retinal pigment epithelial cells (H-RPE) reflects their health and function. Presented here is an optimized protocol for examining the real-time metabolic flux of H-RPE using high-resolution respirometry.