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Guangzhou Women and Children’s Medical Center

3 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice
Ming Fu 1, Zefeng Lin 2, Huiting Lin 2, Yanlu Tong 2, Hezhen Wang 2, Hongjiao Chen 2, Yan Chen 2, Ruizhong Zhang 2
1First Affiliated Hospital of Jinan University, 2Department of Pediatric Surgery, Guangzhou Women & Children's Medical Center, Guangzhou Medical University

This article describes in detail a method based on silver nanoparticles for ameliorating biliary atresia syndrome in an experimental biliary atresia mouse model. A solid understanding of the reagent preparation process and the neonatal mouse injection technique will help familiarize researchers with the method used in neonatal mouse model studies.

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Medicine

A Neonatal BALB/c Mouse Model of Necrotizing Enterocolitis
Yan Tian *1, Junyu Huang *1, Ming Fu 1, Qiuming He 1, Jiale Chen 1, Yan Chen 1, Ruizhong Zhang 1, Wei Zhong 1
1Provincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University

Necrotizing enterocolitis (NEC) is the most severe gastrointestinal (GI) disease that often occurs in premature infants, especially very low birth weight infants, with high mortality and unclear pathogenesis. The cause of NEC may be related to inflammatory immune regulatory system abnormalities. An NEC animal model is an indispensable tool for NEC disease immune research. NEC animal models usually use C57BL/6J neonatal mice; BALB/c neonatal mice are rarely used. Related studies have shown that when mice are infected, Th2 cell differentiation is predominant in BALB/c mice compared to C57BL/6J mice. Studies have suggested that the occurrence and development of NEC are associated with an increase in T helper type 2 (Th2) cells and are generally accompanied by infection. Therefore, this study used neonatal BALB/c mice to induce an NEC model with similar clinical characteristics and intestinal pathological changes as those observed in children with NEC. Further study is warranted to determine whether this animal model could be used to study Th2 cell responses in NEC.

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Immunology and Infection

A Mouse Model of Chronic Liver Fibrosis for the Study of Biliary Atresia
Xinrui Wang 1,2, Ruizhong Zhang 2, Zefeng Lin 2, Ming Fu 2, Yan Chen 1,2
1School of Medicine, South China University of Technology, 2Provincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children’s Medical Center

We established a mouse model of chronic liver fibrosis, which provides a suitable animal model for the virus-induced liver fibrosis mechanistic study of biliary atresia (BA) and a platform for future BA treatments.

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