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University of Agriculture in Kraków

1 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
Aneta Babula *1,2, Izabela Siemińska *1,3, Monika Baj-Krzyworzeka 1
1Department of Clinical Immunology, Jagiellonian University Medical College, 2Doctoral School of Medical and Health Sciences, Jagiellonian University, 3Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU, University of Agriculture in Kraków

The proposed protocol includes guidelines on how to avoid contamination with endotoxin during the isolation of extracellular vesicles from cell culture supernatants, and how to properly evaluate them.

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