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Yonsei University, Mirae Campus

3 ARTICLES PUBLISHED IN JoVE

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Neuroscience

A Molecular Readout of Long-term Olfactory Adaptation in C. elegans
Chao He 1, Jin I. Lee 2, Noelle L'Etoile 3, Damien O'Halloran 1
1Department of Biological Sciences and Institute for Neuroscience, George Washington University, 2Fred Hutchinson Cancer Research Center, 3Department of Cell and Tissue Biology, University of California San Francisco

Here we describe a molecular readout of long-term olfactory adaptation in Caenorhabditis elegans. The Protein Kinase G, EGL-4, is necessary for stable adaptation responses in the primary sensory neuron pair called AWC. During prolonged odor exposure EGL-4 translocates from the cytosol to nucleus of the AWC.

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Biology

Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory
Tong Y. Lee 1, Kyoung-hye Yoon 1, Jin I. Lee 1
1Division of Biological Science and Technology, Yonsei University

We present a simple method to construct 3D nematode cultivation systems called NGT-3D and NGB-3D. These can be used to study nematode fitness and behaviors in habitats that are more similar to natural Caenorhabditis elegans habitats than the standard 2D laboratory C. elegans culture plates.

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Behavior

A Smartphone-Based Imaging Method for C. elegans Lawn Avoidance Assay
Saebom Kwon 1, Jin I. Lee 2, Kyoung-hye Yoon 1
1Department of Physiology, Mitohormesis Research Center, Yonsei University Wonju College of Medicine, 2Division of Biological Science and Technology, College of Science and Technology, Yonsei University, Mirae Campus

This article describes a simple, low-cost method of recording the lawn avoidance behavior of Caenorhabditis elegans, using readily available items such as a smartphone and a light emitting diode (LED) light box. We also provide a Python script to process the video file into a format more amenable for counting.

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