University of Salamanca
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice
Yaneri A. Ayala 1, David Pérez-González 1, Daniel Duque 1,2, Alan R. Palmer 3, Manuel S. Malmierca 1,4
1Auditory Neuroscience Laboratory, Institute of Neuroscience of Castilla y León, University of Salamanca, 2Neural Systems Laboratory, Institute for Systems Research, University of Maryland, 3Medical Research Council Institute of Hearing Research, 4Department of Cell Biology and Pathology, Faculty of Medicine, University of Salamanca
We present methods for the construction of electrodes to simultaneously record extracellular neural activity and release multiple neuroactive substances at the vicinity of the recording sites in awake mice. This technique allows the detailed analysis of putative local synaptic inputs to the neuron of interest.
Behavior
Stereotactically-guided Ablation of the Rat Auditory Cortex, and Localization of the Lesion in the Brain
Verónica Lamas 1,2, Sheila Estévez 1, Marianni Pernía 1, Ignacio Plaza 1, Miguel A. Merchán 1
1Institute of Neuroscience of Castilla y León, University of Salamanca, 2Eaton-Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Department of Otolaryngology, Harvard Medical School
We describe a method for the stereotactically-guided location, exposure, and ablation of the auditory cortex in rats. The localization of the ablation is assessed using a coordinate map postmortem.
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