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University of Idaho

3 ARTICLES PUBLISHED IN JoVE

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Environment

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
Joseph B. DeShields 1, Rachel A. Bomberger 1, James W. Woodhall 2, David L. Wheeler 1, Natalia Moroz 1, Dennis A. Johnson 1, Kiwamu Tanaka 1
1Department of Plant Pathology, Washington State University, 2Parma Research and Extension Center, University of Idaho

Rapid and accurate detection of plant pathogens on-site, especially soil-borne pathogens, is crucial to prevent further inoculum production and proliferation of plant diseases in the field. The method developed here using a portable real-time PCR detection system enables on-site diagnosis under field conditions.

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Biochemistry

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
Joanna Kud 1, Wenjie Wang 2, Yulin Yuan 2, Allan Caplan 2, Joseph C. Kuhl 2, Louise-Marie Dandurand 1, Fangming Xiao 2
1Department of Entomology, Plant Pathology and Nematology, University of Idaho, 2Department of Plant Sciences, University of Idaho

The goal of this manuscript is to present an outline for the comprehensive biochemical and functional studies of the RING-type E3 ubiquitin ligases. This multistep pipeline, with detailed protocols, validates an enzymatic activity of the tested protein and demonstrates how to link the activity to function.

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Immunology and Infection

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
Travis J. Chiarelli 1, Nicole A. Grieshaber 1, Scott S. Grieshaber 1
1Department of Biological Sciences, University of Idaho

This protocol utilizes fluorescent promoter-reporters, live-cell microscopy, and individual inclusion extraction in a directed forward genetic approach to identify and isolate developmental mutants of Chlamydia trachomatis.

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