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Aix Marseille University

7 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Isolating Nasal Olfactory Stem Cells from Rodents or Humans
Stéphane D. Girard 1,2, Arnaud Devéze 3, Emmanuel Nivet 1,2,4, Bruno Gepner 5, François S. Roman 2, François Féron 1,6
1NICN, Aix Marseille University, 2LNPM, Aix Marseille University, 3ENT Department, Aix Marseille University, 4Gene expression Laboratory, The Salk Institute for Biological Studies, 5Laboratory of Speech and Language, Aix Marseille University, 6Centre d'Investigations Cliniques en Biothérapie, Aix Marseille University

We describe here a method for biopsying olfactory mucosa from rat and human nasal cavities. These biopsies can be used for either identifying molecular anomalies in brain diseases or isolating multipotent adult stem cells that can be utilized for cell transplantation in animal models of brain trauma/disease.

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Medicine

Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis
Keith K. Fenrich 1, Pascal Weber 1, Genevieve Rougon 1,2, Franck Debarbieux 1,2
1Developmental Biology Institute of Marseille-Luminy (IBDML), Aix Marseille University, 2Campus de la Timone, European Research Center for Medical Imaging (CERIMED)

We describe a method for implanting and maintaining glass windows over the exposed spinal cords of adult mice for studying experimental autoimmune encephalomyelitis. These windows provide chronic optical access to the spinal cord for monitoring cell dynamics at the subcellular level in live animals using two-photon microscopy.

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Medicine

An Orthotopic Glioblastoma Mouse Model Maintaining Brain Parenchymal Physical Constraints and Suitable for Intravital Two-photon Microscopy
Clément Ricard 1,2, Fabio Stanchi 1,3, Geneviève Rougon 1,2, Franck Debarbieux 1,2
1Developmental Biology Institute of Marseille, Aix Marseille University, 2European Research Center for Medical Imaging, Campus de la Timone, 3Vesalius Research Center, KU Leuven Campus Gasthuisberg

We have established a cortical orthotopic glioblastoma model in mice for intravital two-photon microscopy that recapitulates the biophysical constraints normally at play during the growth of the tumor. A chronic glass window replacing the skull above the tumor enables the follow-up of the tumor progression over time by two-photon microscopy.

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Immunology and Infection

Intravital Microscopy Imaging of the Liver following Leishmania Infection: An Assessment of Hepatic Hemodynamics
Sreekanth Dasari 1, Pascal Weber 1, Camélia Makhloufi 1, Erica Lopez 2, Claire-Lise Forestier 1
1INSERM 1095, URMITE, University of Aix-Marseille, 2U.F.R de Médecine, University of Aix-Marseille

This article reports on a detailed method for the dynamic measurement and quantification of blood flow velocity within individual blood vessels of the mouse liver vasculature using intravital microscopy imaging in combination with a specific methodology for image acquisition and analysis.

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Immunology and Infection

A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis
Jacques Yaacoub Bou Khalil 1, Julien Andreani 1, Didier Raoult 1,2, Bernard La Scola 1,2
1Faculty of Medicine and Pharmacy, Research Unit for Infectious and Tropical Emerging Diseases, Aix Marseille University, 2Pole of Infectious and Tropical Diseases, Clinical and Biological Sector, Federation of Bacteriology-Hygiene Virology, University Hospital Institute Mediterranean Infection

We describe here the latest advances in viral isolation for the characterization of new genotypes of Faustovirus, a new asfarvirus-related lineage of giant viruses. This protocol can be applied to the high throughput isolation of viruses, especially giant viruses infecting amoeba.

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Biology

Single Cell Micro-aspiration as an Alternative Strategy to Fluorescence-activated Cell Sorting for Giant Virus Mixture Separation
Dehia Sahmi-Bounsiar 1, Paulo Victor de Miranda Boratto 2, Graziele Pereira Oliveira 2, Jacques Yaacoub Bou Khalil 1, Bernard La Scola 1,2, Julien Andreani 2
1Institut Hospitalo-Universitaire (IHU) - Méditerranée Infection, 2Microbes, Evolution, Phylogeny and Infection (MEΦI), Aix-Marseille Université UM63, Institut de Recherche pour le Développement IRD 198, Assistance Publique - Hôpitaux de Marseille (AP-HM)

Here, we describe a single cell micro-aspiration method for the separation of infected amoebae. In order to separate viral subpopulations in Vermamoeba vermiformis infected by Faustoviruses and unknown giant viruses, we developed the protocol detailed below and demonstrated its ability to separate two low-abundance novel giant viruses.

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Cancer Research

Flexible Organic Electronic Devices for Pulsed Electric Field Therapy of Glioblastoma
Marie C. Lefevre 1, Attila Kaszas 1, Gerwin Dijk 1,2, Martin Baca 1, Olivier Baudino 2, Bastien Marchiori 2, Loig Kergoat 2, David Moreau 1, Franck Debarbieux 3,4,5, Rodney P. O'Connor 1
1Centre CMP, Bioelectronics Department, Mines Saint-Etienne, 2Panaxium SAS, 3Institut Universitaire de France, 4Institut des Neurosciences de la Timone (UMR7289), CNRS, Aix-Marseille Université, 5Centre Européen de Recherche en Imagerie Médicale, Aix-Marseille Université

This work describes the development of flexible interdigitated electrodes for implementation in 3D brain tumor models, namely, in vitro culture, in ovo model, and in vivo murine model. The proposed method can be used to evaluate the effects of pulsed electric fields on tumors at different levels of complexity.

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