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Italian Institute of Technology

1 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons
Anna Boccaccio 1,2, Claudia Sagheddu 1, Anna Menini 1,3
1SISSA, International School for Advanced Studies, 2Istituto di Biofisica, Consiglio Nazionale delle Ricerche, 3SISSA Unit, Italian Institute of Technology

Photolysis of caged compounds allows the production of rapid and localized increases in the concentration of various physiologically active compounds. Here, we show how to obtain patch-clamp recordings combined with photolysis of caged cAMP or caged Ca for the study of olfactory transduction in dissociated mouse olfactory sensory neurons.

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