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Koc University

2 ARTICLES PUBLISHED IN JoVE

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Biology

Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel
Olgu Enis Tok 1,2, Gamze Demirel 2,3, Yusuf Saatci 1, Zeynep Akbulut 3,5, Ozgecan Kayalar 6, Ranan Gulhan Aktas 2,3,4
1Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, 2Cellorama, 3Cancer and Stem Cell Research Center, Maltepe University, 4Department of Histology and Embryology, School of Medicine, Maltepe University, 5Department of Medical Biology, School of Medicine, Maltepe University, 6School of Medicine, Koc University

The present study describes methodologies for culturing, freezing, thawing, processing, staining, labeling, and examining entire spheroids and organoids under various microscopes, while they remain intact in a hydrogel within a multipurpose device.

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Developmental Biology

Left Atrial Ligation in the Avian Embryo as a Model for Altered Hemodynamic Loading During Early Vascular Development
Bortecine Sevgin *1, Merve Nur Coban *1, Faruk Karatas 2, Kerem Pekkan 1,2
1Department of Biomedical Sciences and Engineering, Koc University, 2Department of Mechanical Engineering, Koc University

Here, we present a detailed visual protocol for executing the left atrial ligation (LAL) model in the avian embryo. The LAL model alters the intracardiac flow, which changes wall shear stress loading, mimicking hypoplastic left heart syndrome. An approach to overcome the challenges of this difficult microsurgery model is presented.

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