Waters Corporation

2 ARTICLES PUBLISHED IN JoVE

Basic Principles for Purification Using Supercritical Fluid Chromatography - ADVERTISEMENT

Jo-Ann M. Jablonski¹, Christopher J. Hudalla¹, Kenneth J. Fountain¹, Steven M. Collier¹, Damian Morrison¹

¹Chemistry Commercial Operations, Waters Corporation

Basic principles for the separation of compounds from mixtures using supercritical fluid chromatography (SFC) are similar to the fundamentals of preparative liquid chromatography.

Chemistry

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Catalin Doneanu^*1, Jing Fang^*1, Yun Alelyunas^*1, Ying Qing Yu¹, Mark Wrona¹, Weibin Chen¹

¹Waters Corporation

Here, we describe a chromatographic assay coupled with the ion mobility separation of peptide precursors followed by the high-resolution (~30,000) MS-detection of peptide fragments for the quantification of spiked peptide standards in a monoclonal antibody digest.