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Pittsburg State University

2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
Michael Taylor 1, Tuhina Banerjee 1, Neyda VanBennekom 1, Ken Teter 1
1Department of Molecular Biology and Microbiology, University of Central Florida

In this report, we describe how surface plasmon resonance is used to detect toxin entry into the host cytosol. This highly sensitive method can provide quantitative data on the amount of cytosolic toxin, and it can be applied to a range of toxins.

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Immunology and Infection

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
Tyler Shelby 1, Shoukath Sulthana 1, James McAfee 1, Tuhina Banerjee 1, Santimukul Santra 1
1Department of Chemistry and Kansas Polymer Research Center, Pittsburg State University

The overall goal of this protocol is to synthesize functional nanosensors for the portable, cost-effective, and rapid detection of specifically targeted pathogenic bacteria through a combination of magnetic relaxation and fluorescence emission modalities.

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