Pittsburg State University

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

Michael Taylor¹, Tuhina Banerjee¹, Neyda VanBennekom¹, Ken Teter¹

¹Department of Molecular Biology and Microbiology, University of Central Florida

In this report, we describe how surface plasmon resonance is used to detect toxin entry into the host cytosol. This highly sensitive method can provide quantitative data on the amount of cytosolic toxin, and it can be applied to a range of toxins.

Immunology and Infection

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

Tyler Shelby¹, Shoukath Sulthana¹, James McAfee¹, Tuhina Banerjee¹, Santimukul Santra¹

¹Department of Chemistry and Kansas Polymer Research Center, Pittsburg State University

The overall goal of this protocol is to synthesize functional nanosensors for the portable, cost-effective, and rapid detection of specifically targeted pathogenic bacteria through a combination of magnetic relaxation and fluorescence emission modalities.