JoVE Logo

Sign In

ETH Zurich

33 ARTICLES PUBLISHED IN JoVE

image

Biology

Chemotactic Response of Marine Micro-Organisms to Micro-Scale Nutrient Layers
Justin R. Seymour 1, Marcos 1, Roman Stocker 1
1Environmental Microfluidics Group, MIT - Massachusetts Institute of Technology

The fabrication of microfluidic channels and their implementation in experiments for studying the chemotactic foraging behaviour of marine microbes within a patchy nutrient seascape and the swimming behaviour of bacteria within shear flow are described.

image

Biology

Studies of Bacterial Chemotaxis Using Microfluidics - Interview
Roman Stocker 1
1Environmental Microfluidics Group, MIT - Massachusetts Institute of Technology

Studies of Bacterial Chemotaxis Using Microfluidics - Interview

image

Bioengineering

Fluorescence detection methods for microfluidic droplet platforms
Xavier Casadevall i Solvas 1, Xize Niu 1, Katherine Leeper 1, Soongwon Cho 1, Soo-Ik Chang 2, Joshua B. Edel 1, Andrew J. deMello 3
1Department of Chemistry, Imperial College London , 2Department of Biochemistry, Protein Chip Research Center, Chungbuk National University, 3Department of Chemistry and Applied Biosciences, Institute for Chemical and Bioengineering, ETH Zurich

Droplet-based microfluidic platforms are promising candidates for high throughput experimentation since they are able to generate picoliter, self-compartmentalized vessels inexpensively at kHz rates. Through integration with fast, sensitive and high resolution fluorescence spectroscopic methods, the large amounts of information generated within these systems can be efficiently extracted, harnessed and utilized.

image

Bioengineering

Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast
Daphne H. E. W. Huberts 1, Georges E. Janssens 2, Sung Sik Lee 3, Ima Avalos Vizcarra 4, Matthias Heinemann 1,5
1Molecular Systems Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 2Department for the Biology of Ageing, European Research Institute for the Biology of Ageing, University Medical Centre Groningen, University of Groningen, 3Institute of Biochemistry, ETH Zurich, 4Department of Health Sciences and Technology, ETH Zurich, 5Institute of Molecular Systems Biology, ETH Zurich

We describe here the operation of a microfluidic device that allows continuous and high-resolution microscopic imaging of single budding yeast cells during their complete replicative and/or chronological lifespan.

image

Medicine

Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer
Rebecca E. Nakles 1, Sarah L. Millman 1, M. Carla Cabrera 1, Peter Johnson 1,2, Susette Mueller 1,2, Philipp S. Hoppe 3, Timm Schroeder 3, Priscilla A. Furth 1,2,4,5
1Department of Oncology, Georgetown University, 2Lombardi Comprehensive Cancer Center, Georgetown University, 3Stem Cell Dynamics, Helmholtz Zentrum München - German Research Center for Environmental Health, 4Department of Medicine, Georgetown University, 5Department of Nanobiomedical Science and WCU Research Center of Nanobiomedical Science, Dankook University

Time-lapse imaging is used to assess behavior of primary preneoplastic mammary epithelial cells derived from genetically engineered mouse models of breast cancer risk to determine if there are correlations between specific behavioral parameters and distinct genetic lesions.

image

Behavior

Two-photon Calcium Imaging in Mice Navigating a Virtual Reality Environment
Marcus Leinweber *1,2, Pawel Zmarz *1,2, Peter Buchmann 3, Paul Argast 1, Mark Hübener 2, Tobias Bonhoeffer 2, Georg B. Keller 1,2
1Friedrich Miescher Institute for Biomedical Research, 2Max Planck Institute of Neurobiology, 3Department of Biosystems Science and Engineering, ETH Zurich

Here we describe the experimental procedures involved in two-photon imaging of mouse cortex during behavior in a virtual reality environment.

image

Environment

Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry
Colin Ruprecht 1, Takayuki Tohge 1, Alisdair Fernie 1, Cara L. Mortimer 2, Amanda Kozlo 2, Paul D. Fraser 2, Norma Funke 1, Igor Cesarino 3,4, Ruben Vanholme 3,4, Wout Boerjan 3,4, Kris Morreel 3,4, Ingo Burgert 5,6, Notburga Gierlinger 5,6, Vincent Bulone 7, Vera Schneider 8, Andrea Stockero 8, Juan Navarro-Aviñó 9, Frank Pudel 10, Bart Tambuyser 11, James Hygate 12, Jon Bumstead 13, Louis Notley 13, Staffan Persson 1,14
1Max Planck Institute for Molecular Plant Physiology, 2School of Biological Sciences, Plant Molecular Science, Centre for Systems and Synthetic Biology, Royal Holloway, University of London, 3Department of Plant Systems Biology, VIB, 4Department of Plant Biotechnology and Bioinformatics, UGhent, 5Institute for Building Materials, ETH Zurich, 6Applied Wood Materials, EMPA, 7Division of Glycoscience, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology (KTH), 8European Research and Project Office GmbH, 9ABBA Gaia S.L., 10Pflanzenöltechnologie, 11Capax Environmental Services, 12Green Fuels, 13Neutral Consulting Ltd, 14Plant Cell Biology Research Centre, School of Botany, University of Melbourne

Plant biomass offers a renewable resource for multiple products, including fuel, feed, food, and a variety of materials. In this paper we investigate the properties of tobacco tree (Nicotiana glauca) and poplar as suitable sources for a biorefinery pipeline.

image

Bioengineering

A Microfluidic Chip for ICPMS Sample Introduction
Pascal E. Verboket 1, Olga Borovinskaya 1, Nicole Meyer 1, Detlef Günther 1, Petra S. Dittrich 1
1Department of Chemistry and Applied Biosciences, ETH Zurich

We present a discrete droplet sample introduction system for inductively coupled plasma mass spectrometry (ICPMS). It is based on a cheap and disposable microfluidic chip that generates highly monodisperse droplets in a size range of 40−60 µm at frequencies from 90 to 7,000 Hz.

image

Behavior

Acquisition of a High-precision Skilled Forelimb Reaching Task in Rats
Ajmal Zemmar *1,2, Brigitte Kast *1,2, Karin Lussi 1,2, Andreas R. Luft *3, Martin E. Schwab *1,2
1Brain Research Institute, University of Zurich, 2Department of Biology and Department of Health Sciences and Technology, ETH Zurich, 3Clinical Neurorehabilitation, Department of Neurology, University of Zurich & University Hospital Zurich

A paradigm is presented to analyze the acquisition of a high-precision skilled forelimb reaching task in rats.

image

JoVE Journal

Speciation and Bioavailability Measurements of Environmental Plutonium Using Diffusion in Thin Films
Ruslan Cusnir 1, Philipp Steinmann 2, Marcus Christl 3, François Bochud 1, Pascal Froidevaux 1
1Institute of Radiation Physics, Lausanne University Hospital, 2Federal Office of Public Health, Bern, Switzerland, 3Laboratory of Ion Beam Physics, ETH Zurich

The technique of diffusive gradients in thin films (DGT) is proposed for speciation studies of plutonium. This protocol describes diffusion experiments probing the behavior of Pu(IV) and Pu(V) in presence of organic matter. DGTs deployed in a karstic spring allow assessment of the bioavailability of Pu.

image

Bioengineering

Easy and Accurate Mechano-profiling on Micropost Arrays
Nils Goedecke 1, Maja Bollhalder 1, Remo Bernet 1, Unai Silvan 1, Jess Snedeker 1,2
1University of Zurich, Balgrist University Hospital, 2Institute for Biomechanics, ETH Zurich

Described are protocols for quantifying mechanical interactions between adherent cells and microstructured substrates. These interactions are closely linked to essential cell behaviors including migration, proliferation, differentiation, and apoptosis. The protocols present an open-source image analysis software called MechProfiler, which enables determination of involved forces for each micropost.

image

Environment

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants
Sarah Heub 1,2, Noe Tscharner 1, Florian Kehl 1,3, Petra S. Dittrich 2, Stéphane Follonier 1, Laurent Barbe 1
1Centre Suisse d'Electronique et de Microtechnique, 2Department of Chemistry and Applied Biosciences, ETH Zurich, 3Department of Information Technology and Electrical Engineering, ETH Zurich

A protocol for the extraction and pre-concentration of estradiol from water samples by using an automated and miniaturized system is presented.

image

Immunology and Infection

In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation
Sarah Stauffer 1,2, Firat Nebioglu 3, Ari Helenius 1
1Institute of Biochemistry, ETH Zurich, 2Department of Biochemistry, University of Zurich, 3Institute for Molecular Health Sciences, ETH Zurich

Disassembly of influenza A virus cores during virus entry into host cells is a multistep process. We describe an in vitro method to analyze the early stages of viral uncoating. In this approach, velocity gradient centrifugation is used to biochemically dissect the steps that initiate uncoating under defined conditions.

image

Chemistry

Characterization, Quantification and Compound-specific Isotopic Analysis of Pyrogenic Carbon Using Benzene Polycarboxylic Acids (BPCA)
Daniel B. Wiedemeier 1, Susan Q. Lang 2, Merle Gierga 3, Samuel Abiven 1, Stefano M. Bernasconi 3, Gretchen L. Früh-Green 3, Irka Hajdas 4, Ulrich M. Hanke 1, Michael D. Hilf 1, Cameron P. McIntyre 4, Maximilian P. W. Scheider 1, Rienk H. Smittenberg 5, Lukas Wacker 4, Guido L. B. Wiesenberg 1, Michael W. I. Schmidt 1
1Department of Geography, University of Zurich, 2Department of Earth and Ocean Sciences, University of South Carolina, 3Department of Earth Sciences, ETH Zurich, 4Laboratory of Ion Beam Physics, ETH Zurich, 5Department of Geological Sciences, Stockholm University

We present the benzene polycarboxylic acid (BPCA) method for assessing pyrogenic carbon (PyC) in the environment. The compound-specific approach uniquely provides simultaneous information about the characteristics, quantity and isotopic composition (13C and 14C) of PyC.

image

Chemistry

Microfluidic Pneumatic Cages: A Novel Approach for In-chip Crystal Trapping, Manipulation and Controlled Chemical Treatment
Afshin Abrishamkar 1,2, Markos Paradinas 3, Elena Bailo 4, Romen Rodriguez-Trujillo 5, Raphael Pfattner 5, René M. Rossi 1, Carmen Ocal 5, Andrew J. deMello 2, David B. Amabilino 6, Josep Puigmartí-Luis 1
1Empa - Swiss Federal Laboratories for Materials Science and Technology, 2Institute of Chemical and Bioengineering, Department of Chemistry and Applied Bioscience, ETH Zurich, 3ICN2-Institut Catala de Nanociencia i Nanotecnologia, 4WITec GmbH, 5Institut de Ciència de Materials de Barcelona, 6School of Chemistry, The University of Nottingham

Herein, we describe the fabrication and operation of a double-layer microfluidic system made of polydimethylsiloxane (PDMS). We demonstrate the potential of this device for trapping, directing the coordination pathway of a crystalline molecular material and controlling chemical reactions onto on-chip trapped structures.

image

Behavior

Investigating Motor Skill Learning Processes with a Robotic Manipulandum
Susan Leemburg 1, Maiko Iijima 1, Olivier Lambercy 2, Lauriane Nallet-Khosrofian 1, Roger Gassert 2, Andreas Luft 1
1Division of Vascular Neurology and Rehabilitation, Department of Neurology, University Hospital Zurich, 2Rehabilitation Engineering Laboratory, Department of Health Sciences and Technology, ETH Zurich

A paradigm is presented for training and analysis of an automated skilled reaching task in rats. Analysis of pulling attempts reveals distinct subprocesses of motor learning.

image

Medicine

An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers
Lukas Kaufmann *1, Mohammedyaseen Syedbasha *1, Dominik Vogt 1, Yvonne Hollenstein 1, Julia Hartmann 1, Janina E. Linnik 1,2,3, Adrian Egli 1,4
1Applied Microbiology Research, Department of Biomedicine, University of Basel, 2Department of Biosystems Science and Engineering, ETH Zurich, 3Swiss Institute of Bioinformatics, 4Clinical Microbiology, University Hospital Basel

The presented protocols describe how to perform a hemagglutination inhibition assay to quantify influenza-specific antibody titers from serum samples of influenza vaccine recipients. The first assay determines optimal viral antigen concentrations by hemagglutination. The second assay quantifies influenza-specific antibody titers by hemagglutination inhibition.

image

Medicine

Echocardiographic and Histological Examination of Cardiac Morphology in the Mouse
Delphine Baudouy 1, Jean-François Michiels 1,2, Ana Vukolic 3, Kay-Dietrich Wagner 1, Nicole Wagner 1
1Université Côte d’Azur, CNRS, INSERM, iBV, 2Department of Pathology, CHU Nice, 3Institute for Molecular Health Sciences, ETH Zurich

Echocardiographic examination is frequently used in mice. Expensive high-resolution ultrasound devices have been developed for this purpose. This protocol describes an affordable echocardiographic procedure combined with histological morphometric analyses to determine cardiac morphology.

image

Chemistry

Microfluidic-based Synthesis of Covalent Organic Frameworks (COFs): A Tool for Continuous Production of COF Fibers and Direct Printing on a Surface
Afshin Abrishamkar 1, David Rodríguez-San-Miguel 2, Jorge Andrés Rodríguez Navarro 3, Romen Rodriguez-Trujillo 4, David B. Amabilino 5, Ruben Mas-Ballesté 2, Félix Zamora 2,6,7, Andrew J. deMello 1, Josep Puigmarti-Luis 1
1Institute of Chemical and Bioengineering, Department of Chemistry and Applied Bioscience, ETH Zurich, 2Departamento de Química Inorgánica, Universidad Autónoma de Madrid, 3Departamento de Química Inorgánica, Universidad de Granada, 4Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), 5School of Chemistry, University of Nottingham, 6Condensed Matter Physics Center (IFMAC), Universidad Autónoma de Madrid, 7Instituto Madrileño de Estudios Avanzados en Nanociencia (IMDEA Nanociencia)

We present a novel microfluidic-based method for synthesis of covalent organic frameworks (COFs). We demonstrate how this approach can be used to produce continuous COF fibers, and also 2D or 3D COF structures on surfaces.

image

Neuroscience

Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System
Bruna Soares Landeira 1, Jéssica Alves de Medeiros Araújo 1, Timm Schroeder 2, Ulrich Müller 3, Marcos R. Costa 1
1Brain Institute, Federal University of Rio Grande do Norte, 2Department of Biosystems Science and Engineering, ETH Zurich, 3The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University

Live imaging is a powerful tool to study cellular behaviors in real time. Here, we describe a protocol for time-lapse video-microscopy of primary cerebral cortex cells that allows a detailed examination of the phases enacted during the lineage progression from primary neural stem cells to differentiated neurons and glia.

image

Bioengineering

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
Richard Bruch *1, André Kling *1,3, Gerald A. Urban 1,2, Can Dincer 1,2
1Department of Microsystems Engineering, University of Freiburg, 2Freiburg Materials Research Center, University of Freiburg, 3Department of Biosystems Science and Engineering, ETH Zurich

A microfluidic biosensor platform was designed and fabricated using low-cost dry film photoresist technology for the rapid and sensitive quantification of various analytes. This single-use system allows for the electrochemical readout of on-chip-immobilized enzyme-linked assays by means of the stop-flow technique.

image

Engineering

Experimental Protocol to Determine the Chloride Threshold Value for Corrosion in Samples Taken from Reinforced Concrete Structures
Ueli M. Angst 1, Carolina Boschmann 1, Matthias Wagner 2, Bernhard Elsener 1,3
1Institute for Building Materials, ETH Zurich, 2Tecnotest AG, 3Department of Chemical and Geological Science, University of Cagliari

We propose a method to measure a parameter that is highly relevant for corrosion assessments or predictions of reinforced concrete structures, with the main advantage of permitting testing of samples from engineering structures. This ensures real conditions at the steel-concrete interface, which are crucial to avoid artifacts of laboratory-made samples.

image

Neuroscience

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations
Rosa Gómez-Villafuertes *1,2,3, Lucía Paniagua-Herranz *1,2,3, Sergio Gascon *4,5, David de Agustín-Durán 1,2,3, María de la O Ferreras 1,2,3, Juan Carlos Gil-Redondo 1,2,3, María José Queipo 1,2,3, Aida Menendez-Mendez 1,2,3, Ráquel Pérez-Sen 1,2,3, Esmerilda G. Delicado 1,2,3, Javier Gualix 1,2,3, Marcos R. Costa 6, Timm Schroeder 7, María Teresa Miras-Portugal 1,2,3, Felipe Ortega 1,2,3
1Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University, 2University Institute for Neurochemistry Research (IUIN), 3Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), 4Institute of Stem Cell Research, Helmholtz Center Munich, Neuherberg/Munich, Germany Physiological Genomics, Biomedical Center, Ludwig-Maximilians University Munich, 5Toxicology and Pharmacology Department, Faculty of Veterinary medicine, Complutense University, 6Brain Institute, Federal University of Rio Grande do Norte, 7Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich

A robust protocol to monitor neural populations by time-lapse video-microscopy followed by software-based post-processing is described. This method represents a powerful tool to identify biological events in a selected population during live imaging experiments.

image

Engineering

Fabrication Procedures and Birefringence Measurements for Designing Magnetically Responsive Lanthanide Ion Chelating Phospholipid Assemblies
Stéphane Isabettini 1, Mirjam E. Baumgartner 1, Peter Fischer 1, Erich J. Windhab 1, Marianne Liebi 2, Simon Kuster 1
1Laboratory of Food Process Engineering, ETH Zurich, 2MAX IV Laboratory, Lund University

Fabrication procedures for highly magnetically responsive lanthanide ion chelating polymolecular assemblies are presented. The magnetic response is dictated by the assembly size, which is tailored by extrusion through nanopore membranes. The assemblies' magnetic alignability and temperature-induced structural changes are monitored by birefringence measurements, a complimentary technique to nuclear magnetic resonance and small angle neutron scattering.

image

Developmental Biology

High Frequency Ultrasound for the Analysis of Fetal and Placental Development In Vivo
Nicole Meyer 1, Thomas Schüler 2, Ana Claudia Zenclussen 1
1Experimental Obstetrics and Gynecology, Medical Faculty, Otto-von-Guericke University, 2Institute of Molecular and Clinical Immunology, Medical Faculty, Otto-von-Guericke University

Here we describe the technique of high frequency ultrasound for in vivo analysis of fetuses in mice. This method allows the follow-up of fetuses and the analysis of placental parameters as well as maternal and fetal blood flow throughout pregnancy.

image

Biochemistry

Quaternary Structure Modeling Through Chemical Cross-Linking Mass Spectrometry: Extending TX-MS Jupyter Reports
Hamed Khakzad 1,2, Swen Vermeul 3, Lars Malmström 4,5,6
1Equipe Signalisation Calcique et Infections Microbiennes, Ecole Normale Supérieure Paris-Saclay, 2Institut National de la Santé et de la Recherche Médicale, 3Scientific IT Services, ETH Zurich, 4Institute for Computational Science, University of Zurich, 5S3IT, University of Zurich, 6Division of Infection Medicine, Department of Clinical Sciences Lund, Faculty of Medicine, Lund University

Targeted cross-linking mass spectrometry creates quaternary protein structure models using mass spectrometry data acquired using up to three different acquisition protocols. When executed as a simplified workflow on the Cheetah-MS web server, the results are reported in a Jupyter Notebook. Here, we demonstrate the technical aspects of how the Jupyter Notebook can be extended for a more in-depth analysis.

image

Education

Fabrication and Design of Wood-Based High-Performance Composites
Marion Frey 1,2, Meri Zirkelbach 3, Clemens Dransfeld 4, Eric Faude 1, Etienne Trachsel 1, Mikael Hannus 5, Ingo Burgert 1,2, Tobias Keplinger 1,2
1Wood Materials Science, Department of Civil, Environmental and Geomatic Engineering, ETH Zurich, 2Cellulose & Wood Materials, Functional Materials, EMPA, 3Design and Arts, Lucerne University of Applied Sciences and Arts, 4Aerospace Manufacturing Technologies, Aerospace Engineering, Delft University of Technology, 5Stora Enso Oyj

Delignified densified wood represents a new promising lightweight, high-performance and bio-based material with great potential to partially substitute natural fiber reinforced- or glass fiber reinforced composites in the future. We here present two versatile fabrication routes and demonstrate the possibility to create complex composite parts.

image

Bioengineering

Generating Controlled, Dynamic Chemical Landscapes to Study Microbial Behavior
Francesco Carrara 1, Douglas R. Brumley 2, Andrew M. Hein 3, Yutaka Yawata 4,5, M. Mehdi Salek 1, Kang Soo Lee 1, Elzbieta Sliwerska 1, Simon A. Levin 6, Roman Stocker 1
1Institute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering, 2School of Mathematics and Statistics, University of Melbourne, 3Institute of Marine Sciences, University of California, Santa Cruz, 4Faculty of Life and Environmental Sciences, University of Tsukuba, 5Microbiology Research Center for Sustainability, University of Tsukuba, 6Department of Ecology and Evolutionary Biology, Princeton University

A protocol for the generation of dynamic chemical landscapes by photolysis within microfluidic and millifluidic setups is presented. This methodology is suitable to study diverse biological processes, including the motile behavior, nutrient uptake, or adaptation to chemicals of microorganisms, both at the single cell and population level.

image

Environment

In Situ Chemotaxis Assay to Examine Microbial Behavior in Aquatic Ecosystems
Estelle E. Clerc 1, Jean-Baptiste Raina 2, Bennett S. Lambert 3, Justin Seymour 2, Roman Stocker 1
1Institute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering, ETH Zürich, 2Climate Change Cluster, University of Technology Sydney, 3School of Oceanography, University of Washington

Presented here is the protocol for an in situ chemotaxis assay, a recently developed microfluidic device that enables studies of microbial behavior directly in the environment.

image

Developmental Biology

Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm
Eishi Aizawa 1, Charles-Etienne Dumeau 1, Anton Wutz 1
1Institute of Molecular Health Sciences, Swiss Federal Institute of Technology, ETH Zurich

This article aims to demonstrate the use of parthenogenetic haploid embryonic stem cells as a substitute for sperm for the construction of semi-cloned embryos.

image

Engineering

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly
Roberto Pioli 1, Roman Stocker 1, Lucio Isa 2, Eleonora Secchi 1
1Institute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering, ETH Zurich, 2Department of Materials, ETH Zurich

We present a technology that uses capillarity-assisted assembly in a microfluidic platform to pattern micro-sized objects suspended in a liquid, such as bacteria and colloids, into prescribed arrays on a polydimethylsiloxane substrate.

image

Environment

A Microfluidic Platform to Study Bioclogging in Porous Media
Dorothee L. Kurz 1,2, Eleonora Secchi 1, Roman Stocker 1, Joaquin Jimenez-Martinez 1,2
1Department of Civil, Environmental and Geomatic Engineering, Institute of Environmental Engineering, ETH Zurich, 2Department Water Resources and Drinking Water, Swiss Federal Institute of Aquatic Science and Technology

The present protocol describes a microfluidic platform to study biofilm development in quasi-2D porous media by combining high-resolution microscopy imaging with simultaneous pressure difference measurements. The platform quantifies the influence of pore size and fluid flow rates in porous media on bioclogging.

image

Bioengineering

Engineering Tendon Assembloids to Probe Cellular Crosstalk in Disease and Repair
Tino Stauber 1,2, Maja Wolleb 1,2, Jess G. Snedeker 1,2
1Department of Orthopedics, Balgrist, University Hospital Zurich, University of Zurich, 2Department of Health Sciences and Technology, Institute for Biomechanics, ETH Zurich

Here, we present an assembloid model system to mimic tendon cellular crosstalk between the load-bearing tendon core tissue and an extrinsic compartment containing cell populations activated by disease and injury. As an important use case, we demonstrate how the system can be deployed to probe disease-relevant activation of extrinsic endothelial cells.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved