JoVE Logo

Sign In

Fraunhofer IME

1 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues
Julia Barthelmes *1, Nadja Tafferner *2, Jennifer Kurz 2, Natasja de Bruin 2, Michael J. Parnham 2, Gerd Geisslinger 1, Susanne Schiffmann 2
1Institute of Clinical Pharmacology, Goethe University Hospital Frankfurt, 2Project Group for Translational Medicine & Pharmacology, Fraunhofer IME

This manuscript describes the methods for induction and scoring of the experimental autoimmune encephalomyelitis (EAE) model, together with the assessment of immune cell distribution and mRNA cytokine levels in lymph nodes, spleen, blood and spinal cord using flow cytometry and quantitative PCR, respectively, at various disease phases.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved