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Miltenyi Biotec GmbH

4 ARTICLES PUBLISHED IN JoVE

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Biology

Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
Melanie Jungblut 1, Karen Oeltze 1, Irene Zehnter 1, Doris Hasselmann 1, Andreas Bosio 1
1Miltenyi Biotec,GmbH

This video describes a simple, time-saving technique for automated tissue dissociation using the gentleMACS Dissociator to prepare single-cell suspensions of mouse splenocytes.

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Biology

Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
Melanie Jungblut 1, Karen Oeltze 1, Irene Zehnter 1, Doris Hasselmann 1, Andreas Bosio 1
1Miltenyi Biotec,GmbH

Dissociating cells from specific tissue types requires specific parameters for tissue agitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on lung tissue is explained.

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Biology

Generation of Single-Cell Suspensions from Mouse Neural Tissue
Sandra Pennartz 1, Sandy Reiss 1, Rebecca Biloune 1, Doris Hasselmann 1, Andreas Bosio 1
1Miltenyi Biotec,GmbH

Dissociating cells from specific tissue types requires specific parameters for tissue aggitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS Dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on nerual tissue is explained.

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Medicine

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells
David J. Agorku 1, Stefan Tomiuk 1, Kerstin Klingner 2, Stefan Wild 1, Silvia Rüberg 1, Lisa Zatrieb 1, Andreas Bosio 1, Julia Schueler 2, Olaf Hardt 1
1R&D Reagents, Miltenyi Biotec GmbH, 2In vivo Tumorbiology, Oncotest GmbH

Human tumor xenografts are vascularized and infiltrated by cells of mouse origin during the growth phase in vivo. To circumvent the bias caused by these contaminating cells during downstream analysis, we developed a method allowing for comprehensive depletion of all mouse cells by magnetic cell sorting.

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