This work was supported by the National Eye Institute (EY015279 and EY028597) and Delaware INBRE (P20 GM103446).

The following protocol was approved by the University of Delaware Institutional Animal Care and Use Committee under protocol #1039-2021-1. In compliance with the Association for Research in Vision and Ophthalmology (ARVO) for the Use of Animals in Ophthalmic and Vision Research24, all ocular survival surgeries can only be performed in one eye. See Table of Materials for details about reagents and instruments used in this protocol. Alternative approaches for extracting the lens fibers&#160;and suturing&#160;are shown in Figure 2&#160; and&#160;Figure 3.
1. Animals
<ol>
	<li>Use any wild-type or genetically modified laboratory mouse strain that has eyes with intact lenses for this procedure.
	<ol>
		<li>Use C57BL/6 mice as a starting point, as their responses are highly reproducible at both the morphological and molecular levels25,26. 
		NOTE: C57BL/6 or any mouse strain can be used. In the video protocol, the FVB mouse is used for better visual demonstration.</li>
		<li>Perform studies of the response of mutants on other mouse strains with strain-matched controls. 
		NOTE: With practice, this procedure may be performed on mice whose lenses have cataracts as long as the lens capsule is intact and the lens has not undergone significant liquefaction. See the study of mice with lens conditional deletion of the transcription factor Sip127.</li>
	</ol>
	</li>
	<li>Perform this surgery on mice of either sex as young as 8 weeks to as old as 24 months25,26.
	<ol>
		<li>Perform this surgery in even younger mice (after eyelid opening) with practice, although it will be more difficult as these eyes will be significantly smaller.</li>
		<li>Take particular care for surgeries on elderly mice due to their lower tolerance for anesthesia25,26. 
		&#8203;NOTE: Sex differences in surgery responses are more prevalent in elderly mice26.</li>
	</ol>
	</li>
</ol>
2. Preparation of solutions and surgical tools
<ol>
	<li>Obtain anesthetizing and analgesic agents, dilation drops, sterile disposable scalpel, #5 Dumont forceps, 2-110 suture forceps, needles (26 G 1/2 and 27 G 45&#176; bent dispensing tip 1&#34;), syringes, and balanced saline solution (BSS).</li>
	<li>Sterilize surgical instruments in an autoclave.</li>
</ol>
3. Prior to anesthesia
<ol>
	<li>Prior to anesthesia, give the mouse one drop each of 1% atropine and 2.5% phenylephrine to dilate the pupil (see Figure 1A) of the eye undergoing surgery. Apply ophthalmic ointment to the non-operated eye to keep the surface from drying. 
	NOTE: The ARVO statement on the use of animals in eye research24 only allows a single eye to undergo survival surgery, leaving the other eye untouched so that the animal retains vision. Thus, survival surgeries are always unilateral; however, the contralateral, unoperated eye can be operated on just prior to sacrifice to create animal-matched &#39;time zero&#39; controls.</li>
	<li>Make sure the eye drops do not run down the mouse&#39;s face and enter the nose, as this can obstruct its breathing.</li>
	<li>Prepare a syringe with sterile BSS using a sterile 26 G 1/2 needle. Fit the syringe with a sterile 27 G 45&#176; bent blunt dispensing tip of 1 inch (referred to as a dispensing tip below).</li>
</ol>
4. Anesthesia
<ol>
	<li>Following eye drops, anesthetize the mouse with an intraperitoneal injection of xylazine/ketamine/acepromazine (35 mg/kg, 80 mg/kg, and 0.5 mg/kg) solution (Figure 1B). 
	NOTE: Inhaled anesthesia might not be possible unless a specialized nose cone can be utilized to allow for access to the eye needed for surgery.</li>
</ol>
5. Making the incision
<ol>
	<li>Once the mouse is completely under anesthesia, as measured by the lack of toe pinch reflex, and its pupil has dilated completely (2 mm) (Figure 4A), the mouse is ready for surgery.
	<ol>
		<li>Move the anesthetized mouse to the stage of a compound dissecting light microscope. Use a heater pad to ensure the mouse has adequate thermal regulation and use sterile drapes.</li>
		<li>View the eye under 10x-20x magnification using a dissecting microscope (see Table of Materials for specifics).</li>
	</ol>
	</li>
	<li>Make a 1-1.5 mm incision in the center of the cornea using a sterile surgical scalpel. Ensure the incision extends through the cornea and incises the anterior lens capsule (Figure 1C and Figure 4B).</li>
</ol>
6. Separating the lens fibers from the capsular bag
<ol>
	<li>Grasp the syringe with a dispensing tip containing sterile BSS and gently expel 1 drop of BSS above the cornea to wet the surface for further manipulation.</li>
	<li>Separate the lens fiber mass from the lens capsule.
	<ol>
		<li>Insert the dispensing tip through the central corneal incision into the lens capsule incision, then gently move the needle in a circular motion while slowly releasing BSS to separate apical-apical connections between lens epithelial and fiber cells (hydro dissection), ultimately expelling the lens fiber mass (Figure 1D).</li>
		<li>In most cases, the fiber mass will eventually adhere to the dispensing tip during this procedure (Figure 2A). If this happens, gently remove the dispensing tip from the inside eye; and clean with a sterile towel to remove this tissue. Repeat until all lens fiber cells are removed.</li>
		<li>Alternatively, use micro forceps (number 5 Dumont) to extract the lens nucleus (Figure 2B). If using this method, be careful that the original corneal incision exceeds the diameter of the nucleus to ensure easy removal.</li>
	</ol>
	</li>
	<li>Rinse the lens capsule with sterile BSS to remove any remaining fibers.</li>
	<li>Confirm that the lens capsule is left inside the eye (Figure 1E and Figure 4C) by viewing its translucent/glassy appearance through the microscope.</li>
</ol>
7. Inflating the anterior chamber and suturing the corneal incision
<ol>
	<li>Inflate the anterior chamber to its normal depth by injecting BSS through the incision into the anterior chamber.
	<ol>
		<li>Add inhibitors or activators of signaling pathways of interest to the BSS to test their roles in the lens injury response12,15. 
		NOTE: Animals can also be treated systemically with pathway inhibitors, which may be needed depending on the pharmacokinetics of drug turnover as the aqueous humor balance is re-established after surgery15.</li>
	</ol>
	</li>
	<li>Suture the corneal incision with square knots using 10-0 nylon thread&#160;with a tapered, curved 5.3 mm 1/2c needle (Figure 1F and Figure 4D-F).
	<ol>
		<li>Place either one square knot stitch in the central part of the incision or two square knot stitches at both the upper and lower halves of the original incision (see Figure 3). Number of stitches depends on the length of the corneal incision.</li>
		<li>Do not puncture both corneal flaps at one time. Instead, hold one corneal flap using forceps in the non-dominant hand and push the needle/nylon through. Once one side of the cornea has been punctured, push the needle/nylon through the next corneal flap. Close the corneal flaps using square knot stitches.</li>
	</ol>
	</li>
</ol>
8. Post-surgical care
NOTE: Topical antibiotic ointment is applied to the eye, and analgesic agents are administered. Mice do not&#160;usually show signs of distress after initial surgery. If needed, slow-release version of buprenorphine could be used.
<ol>
	<li>Immediately following this surgery, do the following sub-steps.
	<ol>
		<li>Apply one drop of 0.5% erythromycin ophthalmic ointment directly above the suture. Make sure the entire incision is covered in ointment.</li>
		<li>Administer an intraperitoneal injection of buprenorphine (0.1 mg/kg).</li>
	</ol>
	</li>
	<li>Allow the mouse to recover from anesthesia in a cage placed on a heating pad to optimize thermal support.&#160; 
	NOTE: Monitor the animal for signs of trauma, distress, or discomfort. Some signs include infection/scratching at the surgical site, displaced suture, loss of fur, etc.</li>
</ol>
9. Monitoring animal recovery
<ol>
	<li>Monitor the animal&#39;s recovery after surgery until it awakes from anesthesia.</li>
	<li>Check mice every day for the first 3 days following surgery. 
	NOTE: It is usually not needed to remove stitches because mice are either sacrificed prior to full healing (2 weeks PCS) or stitches spontaneously fall out.</li>
	<li>Administer buprenorphine orally, as needed for pain management after 4-8 h post-surgery, at a dosage of 0.5 mg/kg (in Jello) or a dosage of 0.1 mg/kg for those not eating. Alternatively, administer buprenorphine extended-release just prior to surgery. 
	NOTE: When possible, preemptive analgesics should be utilized, yet this may require adjustments to the analgesic protocol that require review and approval from the institutional animal care and use committee.</li>
</ol>
10. Euthanasia
<ol>
	<li>Euthanize the mouse at a desired time post-surgery
	<ol>
		<li>Removed the operated eye using the unoperated eye as control tissue; or re-anesthetize the animal, perform lens fiber cell removal surgery on the previously unoperated contralateral eye, then immediately sacrifice the animal (without allowing it to awake from anesthesia) to provide a time zero operated control.</li>
		<li>Ensure quick dissection and tissue harvest. 
		NOTE: LECs undergo transcriptomic changes within minutes of surgery so rapid tissue harvest and fixation/freezing are necessary when studying early LEC responses to surgery.</li>
	</ol>
	</li>
</ol>
11. Performing further analysis on harvested lens tissues
<ol>
	<li>Use the obtained tissue for immunostaining, RNA sequencing, cell culture, or&#160;western analysis12,15,23,25,26,28. Flow cytometry is theoretically possible as well12, although the small number of LECs in the mouse lens (~40,000)29 and the presence of capsule associated cell debris following surgery make this challenging.</li>
	<li>Immunostaining
	<ol>
		<li>Euthanize the mouse in a chamber via CO2 inhalation (4 L/min) from a gas cylinder. Increase the flow rate to 8-10 L/min once the mouse is unconscious and continue this exposure until complete cessation of breathing plus 1 min. 
		NOTE: The mouse can be euthanized using an appropriate method per AVMA Guidelines for the Euthanasia of Animals or per Institutional Animal Care and Use Committee approved guidelines or policies.</li>
		<li>Ensure proper sacrifice by employing a secondary method of euthanasia, e.g., cervical dislocation. 
		NOTE: The Duncan lab has investigated time points ranging from minutes to months PCS12,15,23.</li>
		<li>After the mouse has been humanely sacrificed, isolate the operated eye for analysis.
		<ol>
			<li>Hold micro forceps in the non-dominant hand to grasp the operated eye while holding micro scissors in the dominant hand.</li>
			<li>Gently clip the optic nerve and muscles anchoring the eye. 
			NOTE: Be particularly careful handling eyes that were recently operated on versus those that have had longer to heal PCS. Recently operated eyes are more structurally fragile.</li>
		</ol>
		</li>
		<li>Embed the dissected eye in OCT media for later cryo-sectioning, immunostaining, and semi-quantitative confocal microscopy28,30.</li>
	</ol>
	</li>
	<li>Isolation of the capsular bag with associated cells for RNA sequencing, cell culture, or western blot analysis
	<ol>
		<li>Euthanize the mouse in a chamber via CO2 inhalation (4 L/min) from a gas cylinder. Increase the flow rate to 8-10 L/min once the mouse is unconscious and continue this exposure until complete cessation of breathing plus 1 min.</li>
		<li>Ensure proper sacrifice by employing a secondary method of euthanasia, e.g., cervical dislocation.</li>
		<li>If an early timepoint PCS (within 1-2 days) is required, either remove stitches prior to tissue extraction or make another incision in the cornea through which to extract the lens capsule. 
		NOTE: At later time points PCS, the stitches will likely have fallen out, so a new incision will be needed.</li>
	</ol>
	</li>
	<li>Locate the translucent lens capsule.
	<ol>
		<li>Gently insert the micro forceps through the corneal opening, grasp the capsule, and remove it from the anterior chamber.</li>
		<li>Place the capsule in a 1.5 mL microcentrifuge tube or culture dish for further analysis.</li>
	</ol>
	</li>
</ol>

Replicating Cataract Surgical Techniques in a Mouse Model Through LEC Extraction

<ol>
	<li>Wormstone, I. M., Wormstone, Y. M., Smith, A. J. O., Eldred, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Posterior+capsule+opacification:+What's+in+the+bag.">Posterior capsule opacification: What's in the bag.</a> Prog Retin Eye Res. 82, 100905 (2021).</li><li>Bassnett, S., Shi, Y., Vrensen, G. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biological+glass:+structural+determinants+of+eye+lens+transparency.">Biological glass: structural determinants of eye lens transparency.</a> Philos Trans R Soc Lond B Biol Sci. 366 (1568), 1250-1264 (2011).</li><li>Danysh, B. P., Duncan, M. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+lens+capsule.">The lens capsule.</a> Exp Eye Res. 88 (2), 151-164 (2009).</li><li>Liu, Y. C., Wilkins, M., Kim, T., Malyugin, B., Mehta, J. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cataracts.">Cataracts.</a> Lancet. 390 (10094), 600-612 (2017).</li><li>Khairallah, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Number+of+people+blind+or+visually+impaired+by+cataract+worldwide+and+in+world+regions,+1990+to+2010.">Number of people blind or visually impaired by cataract worldwide and in world regions, 1990 to 2010.</a> Invest Ophthalmol Vis Sci. 56 (11), 6762-6769 (2015).</li><li>Chan, E., Mahroo, O. A., Spalton, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Complications+of+cataract+surgery.">Complications of cataract surgery.</a> Clin Exp Optom. 93 (6), 379-389 (2010).</li><li>Taravati, P., Lam, D. L., Leveque, T., Van Gelder, R. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Postcataract+surgical+inflammation.">Postcataract surgical inflammation.</a> Curr Opin Ophthalmol. 23 (1), 12-18 (2012).</li><li>Shihan, M. H., Novo, S. G., Duncan, M. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cataract+surgeon+viewpoints+on+the+need+for+novel+preventative+anti-inflammatory+and+anti-posterior+capsular+opacification+therapies.">Cataract surgeon viewpoints on the need for novel preventative anti-inflammatory and anti-posterior capsular opacification therapies.</a> Curr Med Res Opin. 35 (11), 1971-1981 (2019).</li><li>El-Harazi, S. M., Feldman, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Control+of+intra-ocular+inflammation+associated+with+cataract+surgery.">Control of intra-ocular inflammation associated with cataract surgery.</a> Curr Opin Ophthalmol. 12 (1), 4-8 (2001).</li><li>Lindstrom, R. L., Galloway, M. S., Grzybowski, A., Liegner, J. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dropless+Cataract+Surgery:+An+Overview.">Dropless Cataract Surgery: An Overview.</a> Curr Pharm Des. 23 (4), 558-564 (2017).</li><li>Assil, K. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dropless+cataract+surgery:+modernizing+perioperative+medical+therapy+to+improve+outcomes+and+patient+satisfaction.">Dropless cataract surgery: modernizing perioperative medical therapy to improve outcomes and patient satisfaction.</a> Curr Opin Ophthalmol. 32 (Suppl 1), S1-S12 (2021).</li><li>Shihan, M. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fibronectin+has+multifunctional+roles+in+posterior+capsular+opacification+(PCO).">Fibronectin has multifunctional roles in posterior capsular opacification (PCO).</a> Matrix Biol. 90, 79-108 (2020).</li><li>Wormstone, I. M., Wang, L., Liu, C. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Posterior+capsule+opacification.">Posterior capsule opacification.</a> Exp Eye Res. 88 (2), 257-269 (2009).</li><li>Awasthi, N., Guo, S., Wagner, B. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Posterior+capsular+opacification:+a+problem+reduced+but+not+yet+eradicated.">Posterior capsular opacification: a problem reduced but not yet eradicated.</a> Arch Ophthalmol. 127 (4), 555-562 (2009).</li><li>Shihan, M. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=&#945;V&#946;8+integrin+targeting+to+prevent+posterior+capsular+opacification.">&#945;V&#946;8 integrin targeting to prevent posterior capsular opacification.</a> JCI Insight. 6 (21), e145715 (2021).</li><li>Wormstone, I. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+human+capsular+bag+model+of+posterior+capsule+opacification.">The human capsular bag model of posterior capsule opacification.</a> Eye (Lond). 34 (2), 225-231 (2020).</li><li>Wormstone, I. M., Collison, D. J., Hansom, S. P., Duncan, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+focus+on+the+human+lens+in+vitro.">A focus on the human lens in vitro.</a> Environ Toxicol Pharmacol. 21 (2), 215-221 (2006).</li><li>Konopi&#324;ska, J., M&#322;ynarczyk, M., Dmuchowska, D. A., Obuchowska, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Posterior+capsule+opacification:+A+review+of+experimental+studies.">Posterior capsule opacification: A review of experimental studies.</a> J Clin Med. 10 (13), 2847 (2021).</li><li>Koopmans, S. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prevention+of+capsule+opacification+after+accommodating+lens+refilling:+pilot+study+of+strategies+evaluated+in+a+monkey+model.">Prevention of capsule opacification after accommodating lens refilling: pilot study of strategies evaluated in a monkey model.</a> J Cataract Refract Surg. 40 (9), 1521-1535 (2014).</li><li>Koopmans, S. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Accommodative+lens+refilling+in+rhesus+monkeys.">Accommodative lens refilling in rhesus monkeys.</a> Invest Ophthalmol Vis Sci. 47 (7), 2976-2984 (2006).</li><li>Call, M. K., Grogg, M. W., Tsonis, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Eye+on+regeneration.">Eye on regeneration.</a> Anat Rec B New Anat. 287 (1), 42-48 (2005).</li><li>Call, M. K., Grogg, M. W., Del Rio-Tsonis, K., Tsonis, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lens+regeneration+in+mice:+implications+in+cataracts.">Lens regeneration in mice: implications in cataracts.</a> Exp Eye Res. 78 (2), 297-299 (2004).</li><li>Novo, S. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+immediate+early+response+of+lens+epithelial+cells+to+lens+injury.">The immediate early response of lens epithelial cells to lens injury.</a> Cells. 11 (21), 3456 (2022).</li><li>. ARVO Statement for the Use of Animals in Ophthalmic and Vision Research Available from: <a target="_blank" href="https://www.arvo.org/About/policies/arvo-statement-for-the-use-of-animals-in-ophthalmic-and-vision-research/">https://www.arvo.org/About/policies/arvo-statement-for-the-use-of-animals-in-ophthalmic-and-vision-research/</a> (2021)</li><li>Faranda, A. P., Shihan, M. H., Wang, Y., Duncan, M. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+aging+mouse+lens+transcriptome.">The aging mouse lens transcriptome.</a> Exp Eye Res. 209, 108663 (2021).</li><li>Faranda, A. P., Shihan, M. H., Wang, Y., Duncan, M. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effect+of+sex+on+the+mouse+lens+transcriptome.">The effect of sex on the mouse lens transcriptome.</a> Exp Eye Res. 209, 108676 (2021).</li><li>Grabitz, A. L., Duncan, M. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Focus+on+molecules:+Smad+Interacting+Protein+1+(Sip1,+ZEB2,+ZFHX1B).">Focus on molecules: Smad Interacting Protein 1 (Sip1, ZEB2, ZFHX1B).</a> Exp Eye Res. 101, 105-106 (2012).</li><li>Shihan, M. H., Novo, S. G., Le Marchand, S. J., Wang, Y., Duncan, M. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+simple+method+for+quantitating+confocal+fluorescent+images.">A simple method for quantitating confocal fluorescent images.</a> Biochem Biophys Rep. 25, 100916 (2021).</li><li>Shi, Y., De Maria, A., Lubura, S., Sikic, H., Bassnett, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+penny+pusher:+a+cellular+model+of+lens+growth.">The penny pusher: a cellular model of lens growth.</a> Invest Ophthalmol Vis Sci. 56 (2), 799-809 (2014).</li><li>Reed, N. A., Oh, D. J., Czymmek, K. J., Duncan, M. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+immunohistochemical+method+for+the+detection+of+proteins+in+the+vertebrate+lens.">An immunohistochemical method for the detection of proteins in the vertebrate lens.</a> J Immunol Methods. 253 (1-2), 243-252 (2001).</li><li>Jiang, J., Shihan, M. H., Wang, Y., Duncan, M. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lens+epithelial+cells+initiate+an+inflammatory+response+following+cataract+surgery.">Lens epithelial cells initiate an inflammatory response following cataract surgery.</a> Invest Ophthalmol Vis Sci. 59 (12), 4986-4997 (2018).</li><li>Mamuya, F. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+roles+of+&#945;V+integrins+in+lens+EMT+and+posterior+capsular+opacification.">The roles of &#945;V integrins in lens EMT and posterior capsular opacification.</a> J Cell Mol Med. 18 (4), 656-670 (2014).</li><li>Manthey, A. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Zeb+proteins+&#948;EF1+and+Sip1+may+have+distinct+functions+in+lens+cells+following+cataract+surgery.">The Zeb proteins &#948;EF1 and Sip1 may have distinct functions in lens cells following cataract surgery.</a> Invest Ophthalmol Vis Sci. 55 (8), 5445-5455 (2014).</li><li>Hupy, M. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Suppression+of+epithelial+to+mesenchymal+transition+markers+in+mouse+lens+by+a+Smad7-based+recombinant+protein.">Suppression of epithelial to mesenchymal transition markers in mouse lens by a Smad7-based recombinant protein.</a> Chem Biol Interact. 344, 109495 (2021).</li></ol>

The Duncan lab has received financial support from Pliantx to test anti-PCO therapies using this surgery model.

This surgical technique requires advanced mouse handling and micro-surgical skills that take practice to develop. The most difficult to master is the placement of fine sutures in the cornea to close the wound. Suppose the experimenter is a total novice to suturing. In that case, it is recommended to first practice using string and cloth, then move to practice using large-diameter sutures on small fruits to master the needed hand movements to make surgical square knots. Then, the experimenter must practice making incision...

The lens is a highly organized, transparent tissue that refracts light to produce a clearly focused image onto the retina1,2,3. This specialized organ is surrounded by an uninterrupted basement membrane (the capsule), which isolates the lens from other parts of the eye. The inner anterior surface of the capsule anchors a monolayer of lens epithelial cells (LECs), which then differentiate at the lens equator into lens fiber cells, which comprise the vast majority of the lens3. A cataract occurs when the lens loses its transparency due to factors like aging, genetic mutation, UV radiation, oxidative stress, and ocular trauma4. Cataract is a leading cause of blindness worldwide, particularly in countries with poor medical care5. However, this condition is now readily treated by surgical removal of opaque lens cells by phacoemulsification in which the central lens capsule and attached lens epithelium are removed, followed by the use of a vibrating probe to break the cellular mass of the lens into smaller fragments that can be suctioned out; leaving behind a capsular bag with some attached equatorial LECs1. Vision is then most commonly restored by post-surgical implantation of an artificial intraocular lens (IOL).
While cataract surgery (CS) is a highly effective and minimally invasive treatment for cataracts, a patient&#39;s post-surgical recovery can be acutely hampered by the development of ocular inflammation5,6,8. This inflammation can cause post-surgical pain, retinal edema leading to retinal detachment, as well as exacerbation of other inflammatory and fibrotic conditions like uveitis and glaucoma4,7,8,9. Ocular inflammation post-CS (PCS) is generally treated with anti-inflammatory eye drops which are plagued by poor patient compliance or dropless cataract surgery that can lead to an increased prevalence of floaters8,10,11. In the long term, the outcomes of CS can be compromised by the development of posterior capsular opacification (PCO)12. PCO occurs when residual LECs left behind after surgery undergo a wound healing response, proliferate, and migrate onto the posterior lens capsule at&#160;months to years PCS contributing to secondary visual obstruction13,14.
Understanding the molecular mechanisms by which CS results in such acute and chronic responses represents a major challenge in the field, as much of the literature investigating PCO relies on the induction of LEC conversion to myofibroblasts in culture via treatment with active transforming growth factor beta (TGF&#946;)12,15. Human capsular bag models created from cadaver lenses cultured in vitro better reflect the biology of the lens PCS as LECs are cultured on their native basement membrane but are difficult to manipulate mechanistically, do not recapitulate the intraocular environment, and are inherently variable due to lens to lens (or donor to donor) variability16,17. Rabbit1,18 and non-human primate19,20&#160;in vivo models of cataract surgery overcome some of these issues but still are not easy to manipulate for mechanistic studies. Notably, a prior study of mammalian lens regeneration found significant lens regeneration within four weeks after lens fiber cells were removed from mice, leaving the lens epithelial cells and capsule behind, while no lens regrowth occurred when the entire lens was removed21,22. We subsequently streamlined this procedure and optimized it for the study of the acute response of LECs to lens fiber cell removal and their subsequent conversion to a mixed population of cells with myofibroblast and fiber cell properties.
Using this mouse model of cataract surgery, we have shown that lens epithelial cells drastically remodel their transcriptome by 6 hours PCS to produce numerous proinflammatory cytokines23, while they begin expressing fibrotic markers as early as 24 h PCS12,23, prior to the onset of canonical TGF&#946; signaling. Mechanistic experiments in knockout mice using this model revealed that cellular fibronectin expression by LECs is required for a sustained fibrotic response PCS, likely due to both its role in the assembly of the fibrotic ECM and cell signaling12. Other studies showed that &#945;V&#946;8-integrin is required for the transition of LECs to myofibroblasts due to its function in TGF&#946; activation, and the potential of this approach to identify anti-PCO therapeutic leads was confirmed as a &#945;V&#946;8-integrin antagonist also blocked LEC EMT15.
Here, we provide a detailed protocol describing how to remove the lens fiber cells from living mice (Figure 1and&#160;Figure 4) while leaving behind the lens capsule and LECs to model the LEC response to cataract surgery.&#160;

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>0.5% Erythryomycin opthalmic ointment USP</td>
			<td>Baush Lomb</td>
			<td>24208-910-55</td>
			<td></td>
		</tr>
		<tr>
			<td>1% Atropine sulfate ophthalmic solution</td>
			<td>Amneal</td>
			<td>60219-1748-2</td>
			<td></td>
		</tr>
		<tr>
			<td>1% Tropicamide opthalmic solution USP</td>
			<td>Akorn</td>
			<td>17478-102-12</td>
			<td></td>
		</tr>
		<tr>
			<td>10-0 Nylon suture</td>
			<td>Ethicon</td>
			<td>7707G</td>
			<td></td>
		</tr>
		<tr>
			<td>2.5% Phenylephrine hydrochloride ophtahlmic solution USP</td>
			<td>Akorn</td>
			<td>17478-200-12</td>
			<td></td>
		</tr>
		<tr>
			<td>26 G 1/2 Needles - straight</td>
			<td>BD PrecisionGlide</td>
			<td>5111</td>
			<td></td>
		</tr>
		<tr>
			<td>27 G 45&#176; bent dispensing tip 1&#34;</td>
			<td>Harfington</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Balanced saline solution</td>
			<td>Phoenix</td>
			<td>57319-555-06</td>
			<td></td>
		</tr>
		<tr>
			<td>Bupranorphine (0.1 mg/kg)</td>
			<td>APP Pharmaceticals</td>
			<td>401730D</td>
			<td></td>
		</tr>
		<tr>
			<td>Chlorhexidine solution</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Needle holder 2-110</td>
			<td>Duckworth &#38; Kent</td>
			<td>2-110-3E</td>
			<td></td>
		</tr>
		<tr>
			<td>Noyes scissors, straight</td>
			<td>Fine Science Tools</td>
			<td>12060-02</td>
			<td></td>
		</tr>
		<tr>
			<td>SMZ800 Nikon model microscope</td>
			<td>Nikon</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Sterile disposable scalpel No.11</td>
			<td>Feather</td>
			<td>2975#11</td>
			<td></td>
		</tr>
		<tr>
			<td>Tweezers #5 Dumont</td>
			<td>Electron Microscopy Sciences</td>
			<td>72700-D</td>
			<td></td>
		</tr>
		<tr>
			<td>Xylazine/Ketamine/Acepromazine (35 mg/kg, 80 mg/kg, 0.5 mg/kg) solution</td>
			<td>APP Pharmaceticals</td>
			<td>401730D</td>
			<td></td>
		</tr>
	</tbody>
</table>

modeling cataract surgery in mice

Cataract surgery (CS) is an effective treatment for cataracts, a major cause of visual disability worldwide. However, CS leads to ocular inflammation, and in the long term, it can result in posterior capsular opacification (PCO) and/or lens dislocation driven by the post-surgical overgrowth of lens epithelial cells (LECs) and their conversion to myofibroblasts and/or aberrant fiber cells. However, the molecular mechanisms by which CS results in inflammation and PCO are still obscure because most in vitro models do not recapitulate the wound healing response of LECs seen in vivo, while traditional animal models of cataract surgery, such as rabbits, do not allow the genetic manipulation of gene expression to test mechanisms. Recently, our laboratory and others have successfully used genetically modified mice to study the molecular mechanisms that drive the induction of proinflammatory signaling and LEC epithelial to mesenchymal transition, leading to new insight into PCO pathogenesis. Here, we report the established protocol for modeling cataract surgery in mice, which allows for robust transcriptional profiling of the response of LECs to lens fiber cell removal via RNAseq, the evaluation of protein expression by semi-quantitative immunofluorescence, and the use of modern mouse genetics tools to test the function of genes that are hypothesized to participate in the pathogenesis of acute sequelae like inflammation as well as the later conversion of LECs to myofibroblasts and/or aberrant lens fiber cells.

Author Spotlight: Unraveling the Molecular Mechanisms in PCO and Fibrosis Following Cataract Surgery

Modeling Cataract Surgery in Mice

Our lab studies the molecular mechanisms underlying posterior capsular opacification, which is the most prevalent negative sequela of cataract surgery. This method has really given us some new fundamental insight into the response of remnant lens epithelial cells to cataract surgery. We found for the very first time that lens epithelial cells produce pro-inflammatory cytokines a day or two before any TGF-beta signaling and the expression of fibrotic genes.And we've taken this even further to show that the expression of alpha-v beta-8 integrin on lens epithelial cells is required for the activation of TGF-beta, leading to later capsular bag fibrosis. And we've taken this even further to show that drugs that block alpha-v beta-8 integrin function block this pathway and prevent the fibrosis, suggesting that this is a new therapeutic target to prevent fibrotic PCO. PCO pathogenesis studies often focus on lens epithelial cell conversion to myofibroblasts via exogenous active TGF-beta.However, this doesn't address how surgery triggers the fibrotic response. This in vivo model allows one to follow how lens epithelial cells respond to injury within the ocular environment, revealing both cell autonomous and non-autonomous mechanisms. We provide a unique in vivo technique for studying lens epithelial cell response to surgery while utilizing advanced genetic tools.In the long term, investigations can elucidate likely therapeutic targets for inflammatory management, while leveraging our finding that alpha-v beta-8 integrin is a likely molecular target to prevent myofibroblast formation post-cataract surgery.

Based on the results from this surgical method, the Duncan Lab has used this mouse model of cataract surgery to construct an injury response time course of lens epithelial cells post-surgery (Figure 5). By 6 h after surgery, 5% of the lens epithelial transcriptome is differentially expressed (Figure 6A), including the upregulation of numerous immediate early response genes and proinflammatory cytokines23 (Figure 6B</s...

Watch this Scientific Journal Video about Unraveling Molecular Mechanisms in PCO and Fibrosis Following Cataract Surgery at JoVE.com

This method models cataract surgery in vivo by removing lens fiber cells from adult mice and leaving behind the capsular bag with attached lens epithelial cells (LECs). The injury response is then assessed at various times post-surgery using molecular and morphological criteria.

Cataract surgery (CS) is an effective treatment for cataracts, a major cause of visual disability worldwide. However, CS leads to ocular inflammation, and ...

Research

JoVE Journal

Biology

Mouse Lens Fiber Cell (LEC) Removal to Mimic the Cataract Surgery

To begin, obtain anesthetizing and analgesic agents, dilation drops, sterile disposable scalpel, desired types of forceps, needles, syringes, and balanced salt solution or BSS. Sterilize the surgical instruments. Prior to anesthesia, administer one drop each of 1%atropine and 2.5%phenylephrine to dilate the pupil of the eye undergoing surgery.Using a sterile 26 gauge half inch needle, prepare a syringe with sterile BSS. Then attach a sterile one inch, 27 gauge blunt dispensing tip bent at 45 degrees to the syringe. Following the mouse's anesthesia, perform a toe pinch to confirm anesthesia depth ensuring pupil dilation reaches two millimeters.Move the anesthetized mouse to the stage of a compound dissecting light microscope with a heating pad. Using a sterile surgical scalpel, make a one to 1.5 millimeter incision in the center of the cornea, ensuring that the anterior lens capsule is also incised. Gently expel one drop of BSS above the cornea to wet the surface for further manipulation.Now, insert the dispensing tip through the central corneal incision into the lens capsule incision, and gently move the needle in a circular motion while slowly releasing BSS to expel the lens fiber mass. Once the fiber mass adheres to the dispensing tip, gently remove the dispensing tip from the inside eye. Clean it with a sterile towel to remove fiber cells and repeat until all lens fiber cells and lens nucleus are removed.Rinse the lens capsule with sterile BSS to remove any remaining fibers. Observe the translucent or glassy appearance to confirm that the capsule is inside the eye. To close the corneal incision, first, hold one corneal flap using forceps in the non-dominant hand, and push the needle with nylon thread through it.Then push it through the next corneal flap. Place one square knot stitch in the central part of the incision and trim the excess thread. Place the bent needle through the corneal gap either above or below the recently placed suture and gently expel BSS to reinflate the eye.For postoperative care, immediately apply one drop of 0.5%erythromycin ophthalmic ointment directly above the suture covering the entire incision. Administer buprenorphine intraperitoneally. Allow the mouse to recover from anesthesia in a cage placed on a heating pad to optimize thermal support and monitor until it recovers.

668 Views.  University of Delaware.  This method models cataract surgery in vivo by removing lens fiber cells from adult mice and leaving behind the capsular bag with attached lens epithelial cells (LECs). The injury response is then assessed at various times post-surgery using molecular and morphological criteria. 

Replicating Cataract Surgical Techniques in a Mouse Model Through LEC Extraction

Summary