Fabrication of Decellularized Spleen Matrix Derived from RatsLifei Yang 1, Yerong Qian 2, Aihua Shi 1, Shasha Wei 1, Xin Liu 4, Yi Lv 1,2,3, Junxi Xiang 2, Peng Liu 3
1National Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, 2Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, 3Center for Regenerative and Reconstructive Medicine, Med-X Institute, The First Affiliated Hospital of Xi'an Jiaotong University, 4Department of Graduate School, Xi'an Medical University
The decellularized spleen matrix (DSM) holds promising applications in the field of liver tissue engineering. This protocol outlines the procedure for preparing rat DSM, which includes harvesting rat spleens, decellularizing them through perfusion, and evaluating the resulting DSM to confirm its characteristics.