Modeling Healthy and Dysbiotic Vaginal Microenvironments in a Human Vagina-on-a-ChipAakanksha Gulati 1, Alicia Jorgenson 1, Abidemi Junaid 1, Donald E. Ingber 1,2,3
1Wyss Institute for Biologically Inspired Engineering, Harvard University, 2Vascular Biology Program and Department of Surgery, Boston Children's Hospital and Harvard Medical School, 3Harvard John A. Paulson School of Engineering and Applied Sciences
This article describes a protocol for creating a microfluidic vagina-on-a-chip (Vagina Chip) culture device that enables the study of human host interactions with a living vaginal microbiome under microaerophilic conditions. This chip can be used as a tool to investigate vaginal diseases as well as to develop and test potential therapeutic countermeasures.
