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The University of Kentucky

1 ARTICLES PUBLISHED IN JoVE

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Cancer Research

Identification of Quiescent Cells in a Zebrafish T-Cell Acute Lymphoblastic Leukemia Model Using Cell Proliferation Staining
Majd A. Al-Hamaly 1,2, Yelena Chernyavskaya 3, Jessica S. Blackburn 2,3
1Pharmacology and Nutritional Sciences, The University of Kentucky, 2Markey Cancer Center, The University of Kentucky, 3Department of Molecular and Cellular Biochemistry, The University of Kentucky

We used cell proliferation staining to identify quiescent cells in the zebrafish T-acute lymphoblastic leukemia model. The stain is retained in non-dividing cells and reduced during cell proliferation, enabling the selection of dormant cells for further interrogation. This protocol provides a functional tool to study self-renewal in the context of cellular quiescence.

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