Accedi

Dong-A University

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

Young Yeon Kim^*1,2, Jee-Hyun Um^*1,2, Jeanho Yun^1,2

¹Department of Biochemistry, College of Medicine, Dong-A University, ²Peripheral Neuropathy Research Center, College of Medicine, Dong-A University

Intracellular ROS has been shown to play an important role in the induction of cellular senescence. Here, we describe a sensitive assay for quantifying ROS levels during cellular senescence. We also provide protocols for assessing the senescence-associated secretory phenotype, which reportedly contributes to various age-related dysfunctions.

Biology

Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima

Jee-Hyun Um^*1,2, Young Yeon Kim^*1,2, Toren Finkel³, Jeanho Yun^1,2

¹Peripheral Neuropathy Research Center, College of Medicine, Dong-A University, ²Department of Biochemistry, College of Medicine, Dong-A University, ³Aging Institute, Department of Medicine, University of Pittsburgh School of Medicine

Mitophagy, the selective degradation of mitochondria, has been implicated in mitochondrial homeostasis and is deregulated in various human diseases. However, convenient experimental methods for measuring mitophagy activity are very limited. Here, we provide a sensitive assay for measuring mitophagy activity using flow cytometry.