Accedi

University of Applied Sciences Kaiserslautern

2 ARTICLES PUBLISHED IN JoVE

Electronic Measurements of Cellular Adhesion Using Field-Effect Transistor Cell-Substrate Impedance Sensing (FETCIS)

Jessica Ka-Yan Law¹, Anna Susloparova^*1, Dieter Koppenhöfer^*1, Xuan Thang Vu¹, Felix Hempel¹, Sven Ingebrandt¹

¹Department of Informatics and Microsystem Technology, University of Applied Sciences Kaiserslautern

Here, we present a protocol to study cellular adhesion using field-effect transistor (FET) devices. This is a label-free and real-time electronic platform complementing the established Electrical Cell-substrate Impedance Sensing (ECIS) method for measurements of adhesion and detachment of confluent cell cultures and is pushing spatial resolution down to single cell.

Developmental Biology

Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization

Silviya Boyadzhieva^*1,2, Sarah C.L. Fischer^*1,2, Svenja Lösch^1,3, Angela Rutz¹, Eduard Arzt^1,2, Klaus Kruttwig¹

¹INM - Leibniz Institute for New Materials, ²Department of Materials Science and Engineering, Saarland University, ³University of Applied Sciences Kaiserslautern

A protocol for the manufacturing process of polymeric thin film composite structures possessing either different Young's moduli or thicknesses is presented. Films are produced for advanced cell culture studies or as skin adhesives.