Accedi

Anhui Normal University

3 ARTICLES PUBLISHED IN JoVE

Biology

RNAi Interference by dsRNA Injection into Drosophila Embryos

Ekaterini Iordanou¹, Rachana R. Chandran¹, Nicholas Blackstone¹, Lan Jiang¹

¹Department of Biological Sciences, Oakland University

RNA interference has been proven very effective to analyze gene function in Drosophila tracheal development. A detailed protocol used by Jiang lab to inject dsRNA into fly embryos to knockdown gene expression is illustrated. This technique has the potential for screening genes required for tissue and organ development in Drosophila.

Education

The Modular Design and Production of an Intelligent Robot Based on a Closed-Loop Control Strategy

Libo Zhang¹, Junjie Zhu², Hao Ren², Dongdong Liu³, Dan Meng⁴, Yanjun Wu¹, Tiejian Luo⁵

¹Institute of Software, Chinese Academy of Sciences, ²Hunan University, ³Fuyang Normal University, ⁴Anhui Normal University, ⁵University of Chinese Academy of Sciences

We present a protocol on modular design and production of intelligent robots to help scientific and technical workers design intelligent robots with special production tasks based on personal needs and individualized design.

JoVE Journal

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Dechun Zhang^*1, Xianzhao Kan^*2, Sarah Elizabeth Huss³, Lan Jiang², Li-Qing Chen³, Yibing Hu⁴

¹Key Laboratory of Three Gorges Regional Plant Genetics and Germplasm Enhancement (CTGU)/Biotechnology Research Center, China Three Gorges University, ²The Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, ³Department of Plant Biology, University of Illinois at Urbana-Champaign, ⁴College of Resources & Environmental Sciences, Nanjing Agricultural University

A method of constructing a phylogenetic tree based on sequence homology of SWEETs from eukaryotes and SemiSWEETs from prokaryotes is described. Phylogenetic analysis is a useful tool for explaining the evolutionary relatedness between homologous proteins or genes from different organism groups.