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3D Health Solutions Inc.

2 ARTICLES PUBLISHED IN JoVE

Medicine

Standardization and Maintenance of 3D Canine Hepatic and Intestinal Organoid Cultures for Use in Biomedical Research

Vojtech Gabriel¹, Christopher Zdyrski¹, Dipak K. Sahoo², Kimberly Dao³, Agnes Bourgois-Mochel², Jamie Kopper², Xi-Lei Zeng⁴, Mary K. Estes⁴, Jonathan P. Mochel^1,3, Karin Allenspach^2,3

Experimental methods to harvest adult stem cells from canine intestinal and hepatic tissues to establish 3D organoid cultures are described. Furthermore, the laboratory techniques to ensure consistent growth and provide standard operating procedures to harvest, biobank, and revive canine intestinal and hepatic organoid cultures are discussed.

Immunology and Infection

Canine Intestinal Organoids in a Dual-Chamber Permeable Support System

Vojtech Gabriel¹, Christopher Zdyrski¹, Dipak K. Sahoo², Kimberly Dao³, Agnes Bourgois-Mochel², Todd Atherly³, Marilyn N. Martinez⁴, Donna A. Volpe⁵, Jamie Kopper², Karin Allenspach^2,3, Jonathan P. Mochel^1,3

¹Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, ²Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, ³3D Health Solutions Inc., ⁴Office of New Animal Drug Evaluation, Center for Veterinary Medicine, Food and Drug Administration, ⁵Division of Applied Regulatory Science, Office of Clinical Pharmacology, Office of Translational Sciences, Center for Drug Evaluation and Research, Food and Drug Administration

Here, we present a protocol describing the culture of canine intestinal organoids in a dual-chamber, permeable support system. Organoid seeding in the permeable supports, the monolayer maintenance, and subsequent drug permeability experiments are described.