Accedi

University Hospital of Wales

2 ARTICLES PUBLISHED IN JoVE

Preparation of Acute Myocardial Tissue Slices from Biopsies of Human Neonates and Infants with Congenital Heart Disease for Physiological Measurements

Moritz Haustein¹, Rewa Nazzal¹, Jan Trieschmann², Gerardus Bennink³, Juergen Hescheler², Frank Pillekamp⁴, Tobias Hannes^1,5

¹Department of Pediatric Cardiology, University Hospital of Cologne, ²Institute for Neurophysiology, University of Cologne, ³Department of Cardio-thoracic Surgery, University Hospital of Cologne, ⁴Department of General Pediatrics, Neonatology and Pediatric Cardiology, University Children's Hospital, Heinrich Heine University, ⁵Children's Hospital for Wales, University Hospital of Wales

Cardiac slices are a unique model for cardiovascular research and bridge the gap between single-cell and whole-heart models. This protocol describes the preparation of viable cardiac slices from myocardial tissue samples excised during surgery for congenital heart disease.

Bioengineering

Transplantation of a 3D Bioprinted Patch in a Murine Model of Myocardial Infarction

Christopher D. Roche^1,2,3,4, Carmine Gentile^1,2,3

¹The University of Sydney, ²University of Technology Sydney (UTS), ³The Royal North Shore Hospital, ⁴University Hospital of Wales

This protocol aims to transplant a 3D bioprinted patch onto the epicardium of infarcted mice modeling heart failure. It includes details regarding anesthesia, the surgical chest opening, permanent ligation of the left anterior descending (LAD) coronary artery and application of a bioprinted patch onto the infarcted area of the heart.