Accedi

California Polytechnic State University (Cal Poly)

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR

Suman K. Pradhan¹, Jerry Guzman¹, Carl Dargitz¹, Stephanie Switalski², Mark Landon¹, Sven Olek³, Bjoern Samans³, Ulrich Hoffmueler³, Uma Lakshmipathy¹

¹Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, ²California Polytechnic State University (Cal Poly), ³Epiontis GmbH, Precision for Medicine

Here we describe a robust method of determining immune cell identity and purity through epigenetic signatures detected using quantitative PCR (qPCR). DNA demethylation at a specific locus serves as a unique identifier for a particular cell type and allows for identification of CD8+, regulatory, or Th17 T cells.