Accedi

Fleischer Institute of Diabetes and Metabolism

1 ARTICLES PUBLISHED IN JoVE

Genetics

Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications

Gabrielle J. Benitez¹, Kosaku Shinoda^1,2,3

¹Departments of Medicine, Albert Einstein College of Medicine, ²Departments of Molecular Pharmacology, Albert Einstein College of Medicine, ³Fleischer Institute of Diabetes and Metabolism

This publication describes a protocol for the isolation of nuclei from mature adipocytes, purification by fluorescence-activated sorting, and single-cell level transcriptomics.