Accedi

Naresuan University

2 ARTICLES PUBLISHED IN JoVE

Biology

Isolation of Retinal Arterioles for Ex Vivo Cell Physiology Studies

Tim M. Curtis^*1, Declan McLaughlin², Michael O'Hare¹, Joanna Kur¹, Peter Barabas¹, Gordon Revolta¹, C. Norman Scholfield³, J. Graham McGeown⁴, Mary K. McGahon^*2

¹Centre for Experimental Medicine, Queen's University of Belfast, ²Centre for Biomedical Sciences (Education), Queen's University of Belfast, ³Department of Pharmaceutical Chemistry and Pharmacognosy, Naresuan University, ⁴School of Medicine, Dentistry and Biomedical Sciences, Queen's University of Belfast

This manuscript describes a straightforward protocol for the isolation of arterioles from the rat retina that can be used in electrophysiological, calcium imaging and pressure myography studies.

Medicine

Isolation of Intrapulmonary Artery and Smooth Muscle Cells to Investigate Vascular Responses

Kittiwoot To-on¹, Usana Chatturong¹, Teerapap Panklai², Iyapa Palang¹, Anjaree Inchan¹, Sutthinee Wisutthathum³, Tamkeen Urooj Paracha⁴, Phapada Apaikawee¹, Krongkarn Chootip¹

¹Department of Physiology, Faculty of Medical Science and Center of Excellence for Innovation in Chemistry, Naresuan University, ²Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University, ³Cosmetics and Natural Products Research Center, Faculty of Pharmaceutical Sciences, Naresuan University, ⁴Snyder Institute for Chronic Diseases, Airways Inflammation Research Group, Department of Physiology and Pharmacology, University of Calgary

Vascular responses of arterial pulmonary circulation can be explored using intrapulmonary artery (IPA) and vascular smooth muscle cells (VSMCs). The present study describes the isolation of IPA in detail and the protocols used for investigating vasorelaxation in response to physiological stimuli.