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Capitolo 15

Metologie biotecnologiche

Cos'è l'ingegneria genetica?
Cos'è l'ingegneria genetica?
Overview Genetic engineering is the process of modifying an organism’s DNA to introduce new, desirable traits. Many organisms, from bacteria to ...
Selezione antibiotica
Selezione antibiotica
Overview Researchers use antibiotic resistance genes to identify bacteria that possess a plasmid containing their gene of interest. Antibiotic resistance ...
DNA ricombinante
DNA ricombinante
Overview Scientists create recombinant DNA by combining DNA from different sources—often, other species—in the laboratory. DNA cloning allows ...
Organismi transgenici
Organismi transgenici
Overview Transgenic organisms are genetically engineered to carry transgenes—genes from a different species—as part of their genome. The ...
Cellule staminali adulte
Cellule staminali adulte
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. ...
Cellule staminali embrionali
Cellule staminali embrionali
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential ...
Induced Pluripotent Stem Cells
Induced Pluripotent Stem Cells
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell ...
Mutagenesi in vitro
Mutagenesi in vitro
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating ...
Isolamento del DNA
Isolamento del DNA
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers ...
Terapia genica
Terapia genica
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy ...
Clonazione riproduttiva
Clonazione riproduttiva
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by ...
CRISPR
CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific ...
DNA Complementare
DNA Complementare
Overview Only genes that are transcribed into messenger RNA (mRNA) are active, or expressed. Scientists can, therefore, extract the mRNA from cells to ...
PCR
PCR
Overview The polymerase chain reaction, or PCR, is a widely used technique for copying segments of DNA. Due to exponential amplification, PCR can produce ...
Genomica
Genomica
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in ...
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