Results: Successful Axenic Fly Rearing Confirmed with CFUs
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Conclusion
Trascrizione
The overall goal of this procedure is to rear fruit flies that are free of associated microorganisms. This method can help any researcher in the Drosophila field control for, manipulate, or study the microbiota, including its relationship to vario
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A method for rearing Drosophila melanogaster under axenic and gnotobiotic conditions is presented. Fly embryos are dechorionated in sodium hypochlorite, transferred aseptically to sterile diet, and reared in closed containers. Inoculating diet and embryos with bacteria leads to gnotobiotic associations, and bacterial presence is confirmed by plating whole-body Drosophila homogenates.