Enzymatic Dissociation of EBs and Hematopoetic Progenitor Isolation
8:23
Endothelial-to-hematopoetic Transition
10:18
T Cell Assay
11:38
Results: Successful Differentiation of hPSCs
14:13
Conclusion
Trascrizione
The overall goal of this method is to direct the Differentiation of Human Pluripotent Stem Cells toward the Primitive or Definitive Hematopoietic Program using a stage specific signal manipulation approach. This method can address many key questio
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Here, we present human pluripotent stem cell (hPSC) culture protocols, used to differentiate hPSCs into CD34+ hematopoietic progenitors. This method uses stage-specific manipulation of canonical WNT signaling to specify cells exclusively to either the definitive or primitive hematopoietic program.